Mean LPS-stimulated cytokine production of IL-1β, IL-2, IL-4,
IL-6, IL-10, IL-17a, TNF-α, MCP-1, and IFN-γ were not significantly different between groups. Mean PHA-stimulated cytokine production of IL-1 β, IL-6, IL-10, and TNF-α were significantly decreased in AC (p<0.05). PHA-stimulated IL-2, IL-4, IL-17a, MCP-1, and IFN-γ were not different. Conclusions: The first 17 subjects in the ZAC trial had increased CK18, insulin resistance, and immune dysfunction. Un-stimulated IL-6, 8, 10 and TNF-α were increased in AC. LPS stimulation induced cytokine production to a similar degree in AC and controls indicating an absence of priming in AC. PHA stimulation failed to induce production of IL-1β, IL-6, IL-10, and TNF-α in AC, but not controls,
suggesting abnormal T-cell function. The potential of zinc therapy NVP-LDE225 chemical structure to correct these biomarkers will be evaluated in the ZAC Trial. Disclosures: Shirish Barve – Speaking and Teaching: Abbott Craig J. McClain – Consulting: Vertex, Gilead, Baxter, Celgene, Nestle, Danisco, Abbott, Genentech; Grant/Research Support: Ocera, Merck, Glaxo SmithKline; Speaking and Teaching: Roche The following people have nothing to disclose: Mohammad K. Mohammad, Keith C. Falkner, Zhanxiang Zhou, Matthew C. Cave Background: Susceptibility to infection and progressive hepatic injury are hallmarks in alcoholic hepatitis (AH), and depressed functions of natural killer (NK) cells and cytotoxic (CD8+) T lymphocytes may be implicated. Both subsets of lymphocytes can directly kill infected or damaged cells through degranulation and/or Rapamycin order the production of IFN-γ, and they may also secrete tissue healing cytokines such as IL-4 and IL-22. The cells are activated through stimulatory receptors e.g. NKG2D on their surface. We, therefore, hypothesized that the cytotoxic cells may be dysactivated or dysfunctional in AH. Methods: We analysed blood samples from 20 severe AH patients, 10 stable alcoholic cirrhosis patients (AC) and 10 healthy controls (HC). We assessed the functionality of NK (CD3-CD56+) and cytotoxic T lymphocytes (CD3+CD8+) in vitro by a flow cytometry based degranulation assay with the human
chronic myeloid leukemia cell line K562. Additionally, we quantified medchemexpress the frequency of IFN-γ, IL-4 and IL-22 producing cells following stimulation and measured the expression of NKG2D. Results: The frequency of cytotoxic T lymphocytes was halved in AH compared with HC (median±IQR: 27.0%±29,9 vs. 56.6±28.1, p=0.005), but we observed no changes in NK cell frequency (10.4%±12.4vs. 14.0%±10.1). Functionally, the NK cells had markedly decreased ability to degranulate compared with both AC (9.4%±3.7 vs. 18.8%±17.3, p=0.04) and HC (14.1%±11.2, p=0.02). In the same way, we detected no up-regulation of the IFN-γ production in either cell subset. Nevertheless, we observed at least a doubling in the frequencies of both NK cells and cytotoxic T lymphocytes that produced IL-4 or IL-22 compared with HC (p<0.05).