Anamorphs reported for genus: coelomycetous with muriform conidia (see Liu
2009). Literature: Cheng et al. 2004; Hino 1961; Kishi et al. 1991; Liu 2009; Morakotkarn et al. 2008. Type species Shiraia bambusicola Henn., Bot. Jb. 28: 274 (1900). (Fig. 88) Fig. 88 Shiraia bambusium (from IFRD 2040). a Ascostroma form a nubby structures on the twigs of host. b Vertical section of an ascostroma. Note the reddish staining of the inner tissue. c, d Cylindrical asci with a short pedicel. e–g Muriform fusoid hyaline ascospores. Scale bars: a = 1 cm, b = 1 mm, c, d = 50 μm, e–g = 20 μm Ascostroma 1–1.5 cm high × 1–2.5 cm diam., subglobose, oblong to irregular, slightly pink with cracking surface. Ascomata 350–800 μm high × 300–700 μm diam., subglobose, gregarious on the surface layer of ascostroma, immersed, ostiolate, with a small black opening seen on the surface of the selleck chemicals llc ascostroma, ostiole rounded, the inner tissue of ascostroma carnation red (Fig. 88a and b). Hamathecium of dense, long trabeculate pseudoparaphyses, 0.8–1.5 μm broad, anastomosing and branching between the asci. Asci 300–425 × 20–35 μm (\( \barx = 360.5 \times 28 \mu \textm \), n = 10), 6-spored, bitunicate, fissitunicate, cylindrical to cylindro-clavate,
with a short furcate pedicel, up to 50 μm long, with a big and truncate ocular chamber (Fig. 88c and d). Ascospores 62.5–80 × 17.5–22.5 μm (\( \barx = 72.3 \times 19.3 \mu \textm \), n = 10), obliquely uniseriate and partially overlapping, narrowly fusoid to fusoid with tapering or narrowly rounded ends, hyaline turning pale brown when mature, selleck inhibitor muriform, with 9–13 transversal septa, 1–3 longitudinal septa in central cells, slightly constricted at the septa, usually with a gelatinous cap at each end (Fig. 88e, f and g). Anamorph: coelomycetous with muriform conidia (see Liu 2009). Material examined: CHINA, Zhejiang, Hangzhou, Panan, on bamboom, 15 Jun. 2009, leg.
Liu Yongxiang (IFRD 2040). Notes Morphology Shiraia is reported as a parasite on branches of several genera of bamboo distributed mainly in southern regions of China and Japan (Hino 1961; Kishi et al. 1991; Liu 2009). Shiraia is characterized by its bambusicolous habitat, large ascostroma and muriform ascospores. Asci comprise 6 ascospores in this study and some previous studies (Hino 1961; Liu 2009). Shiraia bambusicola is Glutathione peroxidase well studied because of its medical effect in anticancer treatment (Kishi et al. 1991). Phylogenetic study Based on the SSU and ITS rDNA sequences analysis, its pleosporalean status was verified, and Shiraia was suggested to be closely related to Leptosphaeriaceae and/or Phaeosphaeriaceae (Pleosporineae) (Cheng et al. 2004). Based on the molecular phylogenetic analysis, another Shiraia-like fungus was reported which produced distinctive prawn-shaped conidioma-like structures (Morakotkarn et al. 2008), and differed from conidiomata in the anamorph of S. bambusicola described by Liu (2009).