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key gene expression differences between the human and bovine pathogens of the Mycobacterium tuberculosis complex. Microbiology 2007,153(Pt 10):3323–3336.CrossRefPubMed Authors’ contributions XZ designed from the database, collected, curated the data and wrote the manuscript. SC analyzed the data and developed the database. KF and SC developed the database and did the programming work. JL, ZW, and XY performed the microarray experiments and analyzed the data. GFG, and HY revised the manuscript. BZ and JW supervised the work, manage the team and wrote the manuscript. All authors read and approved the final manuscript.”
“Background Most of the commonly found structural changes in DNA are due to methylation of selected bases. In some viral DNAs, certain bases may be hydroxymethylated or glucosylated [1–3]. Altered or unusual bases in DNA molecules often have significant physiological implications, such as DNA replication control, gene regulation, or protection of the respective organisms from invasion by foreign DNA . In contrast to other types of DNA modification, S, lividans has a site-specific and stereo-selective sulfur modification on the DNA backbone termed phosphorothioation [5–7]. This sulfur modification occurs specifically between two guanine nucleotides in S.lividans [6, 8]. The sulfur-modified DNA suffers double-stranded cleavage at the modification sites during normal and pulsed-field gel electrophoresis [6, 9–13].