Changes in biochemical parameters under induced blight stress as compared with uninoculated control were less in resistant genotypes than that in susceptible genotype. The deviations in biochemical contents were highest in susceptible genotype N-118. Based on the variations of above parameters under stress and non-stress control among
the four tested genotypes, the overall pattern of changes was N-118 > Duradim > Jhankri > DP-25, which is in accordance with the pattern of increasing resistance. The resistant genotypes could be used for commercial cultivation and genetic improvement programme to develop resistant varieties to Phytophthora leaf blight disease. “
“SYBR Green real-time RT-PCR assay was developed and optimized for the sensitive detection of Onion yellow dwarf virus (OYDV), Leek selleck compound yellow stripe virus (LYSV), Garlic common latent virus (GCLV), Shallot latent virus (SLV) and Mite-borne filamentous virus (MbFV). The polyvalence of the designed primers was tested on 50 genotypes of
garlic (Allium sativum L.) which originated from different countries. Plasmid standards were prepared and used as positive standards. The efficiencies of all reactions were 97, 93, 99, 98 and 87% for OYDV, LYSV, SLV, BMN673 GCLV and MbFV standards, respectively. The detection limit for OYDV, LYSV and GCLV was as low as five gene copies, for SLV it was 15 gene copies
and for MbFV it was 130 gene copies. In comparison with ELISA, more virus-positive garlic accessions were detected with LYSV and GCLV by SYBR Green-based real-time RT-PCR assay. This method was shown to be a more suitable tool for the detection of highly variable pathogens, such as garlic viruses. “
“Symptomatic tomato plants exhibiting big bud, proliferation Tacrolimus (FK506) and small leaves of lateral shoots, purplish top leaves, phyllody, enlarged pistils, hypertrophic calyxes and small and polygonal fruit were collected in Yunnan Province of China. Pleomorphic phytoplasma-like bodies were observed in the phloem sieve tube elements of symptomatic plants by transmission electron microscopy. The presence of phytoplasma in collected samples was further analysed and identified by PCR and virtual computer-simulated restriction fragment length polymorphism (virtual RFLP). A 1.2 kb product was amplified by PCR with universal primers R16F2n/R16R2. Sequence comparisons revealed that the tested strains shared 99% 16S rRNA gene sequence similarity with members of ‘Candidatus Phytoplasma aurantifolia’ (16SrII group). Phylogenetic and virtual RFLP analysis of the 16S rRNA gene sequences confirmed that the phytoplasma is a member of the 16SrII group. This is the first report of 16SrII group phytoplasma infecting tomato in China.