2%), ‘microbi’ OSI-744 (3.2%), ‘lake’ (1.9%), ‘water’ (1.7%) and ‘depth’ (1.6%) (246 hits in total). The most frequently occurring keywords within the labels of environmental samples which yielded hits of a higher score than the highest scoring species were ‘sediment’ (5.4%), ‘microbi’ (2.5%), ‘lake’ (2.1%), ‘water’ (1.9%) and ‘contamin’ (1.8%) (152 hits in total). These keywords reflect some of the ecological and properties reported for strain ASRB2T in the original description [1]. Figure 1 shows the phylogenetic neighborhood of D. acetoxidans in a 16S rRNA based tree. The sequence of the single 16S rRNA gene in the genome differs by 20 nucleotides from the previously published 16S rRNA sequence (“type”:”entrez-nucleotide”,”attrs”:”text”:”AF002671″,”term_id”:”4100902″,”term_text”:”AF002671″AF002671), which contains eleven ambiguous base calls Figure 1 Phylogenetic tree highlighting the position of D.

acetoxidans relative to the type strains of the other species within the order Syntrophobacterales. The tree was inferred from 1,457 aligned characters [6,7] of the 16S rRNA gene sequence under the maximum … Cells of strain ASRB2T are oval to rod-shaped with a size of 1.3 x 1.9-2.2 ��m, appear singly or in pairs (Figure 2) and occasionally contain gas vacuoles in the late-exponential growth phase [1]. The strain is non-motile, non-spore-forming and stains Gram-negative (Table 1) [1]. Strain ASRB2T has a temperature range for growth between 27 and 47��C, with an optimum at 36-40��C [1]. At the optimum growth temperature with acetate as sole carbon and energy source the shortest doubling time recorded was 1.

7-2.2 days [1]. Growth rate in brackish medium was significantly (4.8 x) slower, and no growth was observed in marine medium [1]. The pH range for growth is 6.5-8.3, with an optimum of pH 7.1-7.5 [1]. Desulfoviridin was not observed, but the c-type cytochromes were present [1]. Sulfate or other inorganic sulfur components serve as electron acceptors via reduction to H2S [1]. Strain ASRB2T degrades acetate (as the common carbon source and electron donor) completely to CO2 via the acetyl-CoA/CO-dehydrogenase pathway [1]. The key enzyme of this pathway is encoded by the genes Desac_1965 �C Desac_1969. Several more putative electron donors were tested but not found to be utilized by strain ASRB2T, such as: propionate, butyrate, lactate, H2/CO2, formate, ethanol, propanol, butanol, pyruvate, fumarate, glucose, crotonate, benzoate, phenol, aspartate and glutamate [1].

Figure 2 Scanning electron micrograph of D. acetocidans ASRB2T Table 1 Classification and general features of D. acetocidans Brefeldin_A ASRB2T according to the MIGS recommendations [14] and the NamesforLife database [15]. Chemotaxonomy No data on cell wall structure, quinones, fatty acid pattern or polar lipids are available for this strain.