, 2002) Many members of this genus of Gram-positive, soil-dwelli

, 2002). Many members of this genus of Gram-positive, soil-dwelling bacteria have atypically large genes (>10 kb in size) encoding multimodular polyketide synthases and nonribosomal peptide synthetases that catalyze the biosynthesis of polyketide and nonribosomal peptide antibiotics, respectively (Bentley et al., 2002). The expression of the extraordinarily large genes encoding these mega-enzymes has long been a curiosity (Lipmann et al., 1971; Schwarzer et al., 2003). Three of the largest genes in

the S. coelicolor genome encode the nonribosomal peptide synthetases CDA PSI, CDA PSII, and CDA PSIII (Bentley et al., 2002). The cdaPSI gene (SCO3230) is the largest gene in S. coelicolor at 22 391 bp (Bentley et al., 2002). cdaPSII (SCO3231) and cdaPSIII (SCO3232) are 11 012 and 7253 bp in size, respectively (Bentley et al., 2002; http://strepdb.streptomyces.org.uk). Selleck Ceritinib The megaenzymes encoded by these genes catalyze the biosynthesis

of a cyclic lipopeptide called the calcium-dependent antibiotic (CDA) (Hopwood, 1979; Hopwood & Wright, 1983; Chong et al., 1998; Hojati et al., 2002). We proposed that any influence of LepA on the translation of the cda transcripts would be evident in the amount of CDA that a S. coelicolor strain produces. Surprisingly, we found that a S. coelicolor lepA null strain produces more CDA than the wild-type strain. Escherichia coli strain selleck chemical DH5α was used as the general cloning host. Escherichia coli BW25113 (pIJ790) was used as a host for λRED recombination (Gust et al., 2003). Escherichia coli ET12567

(pUZ8002) was used as the donor in conjugations with S. coelicolor M600 (SCP1−, SCP2−), a plasmid-free derivative of wild-type S. coelicolor A3(2) (Kieser et al., 2000; Gust et al., 2003). Bacillus mycoides was used for CDA bioassays. Escherichia coli strains were grown in Luria–Bertani broth or on agar supplemented with antibiotics [ampicillin (100 μg mL−1), apramycin (50 μg mL−1), chloramphenicol (25 μg mL−1), hygromycin (75 μg mL−1), and kanamycin (50 μg mL−1)]. The media for growth of Streptomyces strains were mannitol soya flour medium, Difco nutrient agar medium (DNA), OXOID nutrient agar medium, liquid yeast extract–malt LY294002 extract medium, 2 × YT, and OXOID nutrient broth (Kieser et al., 2000). As was necessary, those media were supplemented with antibiotics [apramycin (50 μg mL−1), hygromycin (40 μg mL−1), kanamycin (50 μg mL−1), and nalidixic acid (20 μg mL−1)]. Bacillus mycoides was grown on soft nutrient agar supplemented with calcium nitrate [Ca(NO3)2] (Kieser et al., 2000). Standard cloning procedures were used in generating the plasmids described in this work (Sambrook & Russell, 2001). pBluescript II KS+ (Stratagene) was used for subcloning. pMS81, a hygromycin-resistant ΦBT1 attP-int-based vector (Gregory et al., 2003), was used for genetic complementation.

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