Methods: Systolic blood pressure(SBP) was measured at 1-week intervals after clipping. Two and 5 weeks after operation, the rats were sacrificed for western-blot and immunohistochemistry. Results: SBP increased in 2K1C rats(n = 12) within 1 week after unilateral renal clipping relative to sham rats(n = 8). Glomerulosclerosis and tubulointerstitial inflammation were aggravated in maintenance phase. From the acute phase, CK showed significant reduction
in ACE2, leading to an increased ratio of ACE/ACE2. Juxtaglomerular(JG) renin was increased in CK and suppressed in NCK, but collecting duct(CD) renin was enhanced in both kidneys beta-catenin inhibitor in immunohistochemistry. In the maintenance phase, medulla in both kidneys presented significantly increased ACE and decreased ACE2, along with up-regulation of renin in medulla of NCK. Immunohistochemistry revealed more intense CD renin staining in both kidneys. Simultaneously AT1R in CK cortex was not suppressed, albeit there was reduced MasR, thus AT1R/MasR ratio was insignificantly elevated in cortex. Conclusion: Even though the reduction of ACE2 along with increase of JG renin in CK could initiate hypertension in the acute phase, eventually a higher stimulation of ACE and suppression of ACE2, according to the activation of CD renin in NCK, are Ibrutinib thought to play key roles for keeping hypertension during
the maintenance phase. In addition, we cautiously presumed the imbalance of AT1R and MasR might have some effects to hypertension in this model. KATSUNO TAKAYUKI, YAMAGUCHI MAKOTO, Dolutegravir purchase TANAKA AKIHITO, YASUDA YOSHINARI, KATO SAWAKO, SATO WAICHI, TSUBOI NAOTAKE, ITO YASUHIKO, MARUYAMA SHOICHI, MATSUO SEIICHI Department of Nephrology, Nagoya University Graduate
School of Medicine Introduction: Albuminuria is known to be a predictive factor for chronic kidney disease (CKD) and cardiovascular disease (CVD). Particularly in the hypertensive patients, albuminuria increases the risk of CKD and CVD. However, little is known about the prevalence of albuminuria among hypertensive patients. The aim of this study is to conduct factual investigation of albuminuria. Methods: The study subjects were 387 individuals who attended a private practice as an outpatient. Semi-quantitative measurement of urinary albumin excretion corrected for the urinary creatinine levels (albumin creatinine ratio: ACR) was conducted by using a urine reagent paper in the hypertensive patients, and cross-sectional analysis was performed. Results: The cohort consisted of 215 males (55.6%) and 172 females (44.4%), with a mean age of 68.3 years (range 28 to 92 years). 367 patients (94.8%) used an antihypertensive agent. 155 patients (40.1%) had a diabetes mellitus. In 57 patients (14.7%) tested, there was evidence of proteinuria by using a test strip. Mean serum creatinine for the entire cohort was 0.83 mg/dL (range 0.4 to 2.1 mg/dL). Among 385 patients, 197 (51.