Quality of

the RNA was analyzed on an Agilent 2100 BioAna

Quality of

the RNA was analyzed on an Agilent 2100 BioAnalyzer using the RNA6000 labchip kit (Agilent Technologies, Palo Alto, CA). Biotin-labeled antisense cRNA was generated by labeling 20 or 2 μg of total RNA with the BioArray High Yield RNA transcription labeling kit (ENZO) or the Affymetrix Eukaryotic One-Cycle Target Labeling and Control Reagent package, respectively. The quality of the cRNA was checked using the Agilent 2100 bioanalyzer. The labeled cRNA was hybridized to Affymetrix A. niger Genechips. Absolute values of expression were calculated from the scanned array using the Affymetrix GCOS software after an automated process of washing and staining. Microarray Suite Affymetrix v5.1 (Affymatrix Inc., Santa Clara, CA), Spotfire DecisionSite (Spotfire Inc, Somerville, MA), https://www.selleckchem.com/products/nct-501.html GeneData Expressionist Analyst V Pro 2.0.18 (GeneData, Basel, Switzerland) and the R statistical environment http://​www.​r-cran.​org were used for data analyses. Acknowledgements Trichostatin A cost The authors would like to thank Dr A.M. Levin and prof. Dr H.A.B. Wösten for providing the data of the A. niger microarray analysis. References 1. Pel HJ, de Winde JH, Archer DB, Dyer PS, Hofmann G, Schaap PJ, Turner G, de Vries RP, Albang R, Albermann K, et al.: Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88. Nature biotechnology 2007, 25:221–231.CrossRefPubMed 2. Tsitsigiannis DI, Kowieski TM, Zarnowski

R, Keller NP: Endogenous lipogenic regulators of spore balance in Aspergillus PF-01367338 clinical trial nidulans. Eukaryot Cell 2004, 3:1398–1411.CrossRefPubMed 3. Tsitsigiannis DI, Kowieski TM, Zarnowski R, Keller NP: Three putative oxylipin biosynthetic genes integrate sexual and asexual development in Aspergillus nidulans. Microbiol 2005, 151:1809–1821.CrossRef 4. Tsitsigiannis DI, Zarnowski R, Keller NP: The lipid body protein, aminophylline PpoA, coordinates sexual and asexual sporulation in Aspergillus nidulans. J Biol Chem 2004, 279:11344–11353.CrossRefPubMed 5. Taylor JW, Jacobson DJ, Fisher

MC: The evolution of asexual fungi: reproduction, speciation and classification. Ann Rev Phytopathol 1999, 37:197–246.CrossRef 6. Dyer PS, Paoletti M: Reproduction in Aspergillus fumigatus : sexuality in a supposedly asexual species? Med Mycol 2005, 43:S7-S14.CrossRefPubMed 7. Wadman MW, van Zadelhoff G, Hamberg M, Visser T, Veldink GA, Vliegenthart JFG: Conversion of linoleic acid into novel oxylipins by the mushroom Agaricus bisporus. Lipids 2005, 40:1163–1170.CrossRefPubMed 8. Calvo AM, Gardner HW, Keller NP: Genetic connection between fatty acid metabolism and sporulation in Aspergillus nidulans. J Biol Chem 2001, 276:25766–25774.CrossRefPubMed 9. Abell BM, Holbrook LA, Abenes M, Murphy DJ, Hills MJ, Moloney MM: Role of the proline knot motif in oleosin endoplasmic reticulum topology and oil body targeting. Plant Cell 1997, 9:1481–1493.CrossRefPubMed 10. Champe SP, El-Zayat AAE: Isolation of a sexual sporulation hormone from Aspergillus nidulans.

Comments are closed.