The SACE and SChao1 value (richness estimators) and number of OTUs are specified on the top of each histogram. MLN2238 datasheet Arbitrarily assigned OTU reference numbers are given in each section of the histogram, and their taxonomic affiliations are presented in the key. The OTUs affiliated to non-pigmented taxa generally dominated the clone libraries (from 67.6% in C + Nut to 85.3% in UV + Nut; Figure 4 and Additional file 2: Table S1). Among them, Ciliates and uncultured Alveolates were generally well represented (accounting from 14 to 32% of total OTUs, and from 13 to 37% of clones, this website according to the treatments). However, the
increase of non-pigmented group proportions within most of the libraries (compared to T0) was mainly linked to the emergence of taxa affiliated to parasitic groups: Hyphochytrids and genus
Pirsonia (Heterokonta), and Amoebophrya (Alveolata). The proportion of these sequences clearly AZD1390 molecular weight increased during the incubation in all types of treatment. Parasitic taxa related to Amoebophrya particularly emerged in treatments with the highest temperatures (T, T + Nut, TUV, and to a lesser extent TUV + Nut), while Hyphochytrids were strongly associated with all other treatments (C, C + Nut, UV, UV + Nut) (Figure 4). The CCA plot illustrates the significant link between the increase in temperature and the presence of numerous sequences affiliated to Amoebophrya, while sequences affiliated to Hyphochytrides have an opposite Thymidylate synthase position in the plot (Figure 5). The potential hosts of Amoebophrya are primarily found within the class Dinophyceae, and it is noticeable that we observed a large number of pigmented Dinophyceae cells infected by parasites (multinucleated parasites in division in the cells) at T96 h in
all types of treatment (data not shown). Pigmented Dinophyceae were indeed favored by the temperature increase but were also strongly positively affected by nutrient addition and UVBR increase (Figure 5). Pigmented Dinophyceae and Amoebophrya were represented by 7 different OTUs each. Even though the presence/absence of these OTUs varied according to the treatments, no association between the abundance of host and parasite OTUs was observed. Figure 5 Correspondence Canonical Analysis (CCA) performed on the sequencing results expressed as proportion of OTUs detected in the eight libraries constructed at T96 h (i.e. C, UV, T, TUV, C + N, UV + N, T + N, TUV + N treatments). Environmental variables are heterotrophic bacteria (Bact), picocynobacteria (Picocyan), viruses (virus), temperature (Temp), UVB radiation (UV), nutrient concentration (Nut).