The properties measured were tensile strength (MPa), elongation at break (%), and Young’s modulus (MPa). The apparent opacity (Yap) of the films was determined using a colorimeter (BYK Gardner, USA) and was calculated based on the ratio between the

luminosity (L*) of the system (CIELab), which was measured with a black background ( LB*) and a white background ( LW*), and the thickness of the film (φ). The results were expressed on an arbitrary scale (0–100% μm−1) according to Equation BMS-354825 chemical structure (1): equation(1) Yap=[(LB*/LW*)/φ]×100 The opacity of the films intercalated with fresh pasta was determined after 2 and 37 days of storage at 10 °C. Water vapour permeability (WVP) was determined gravimetrically, according to the ASTM E96-00 (1996) and under a relative

humidity gradient of 33–75%. The tests were conducted in duplicate. The yeast and mould counts in fresh pasta were taken in Dichloran Rose Bengal Chloramphenicol (DRBC) agar incubated at 24 °C for 5–7 days; coliform bacteria, which were grown at 45 °C, were counted using the most probable number method (MPN) (APHA, 2001, p. 676). The water activity of the fresh pasta was determined using an Aqualab CX2T equipment (Decagon Devices, USA) at 25 ± 2 °C, and the moisture content (on a wet basis) was determined according to the procedure described in the AOAC 925.04 (1995). Analyses were performed in duplicate. The colour parameters L*, a* and b* (CIELab system)

of the fresh CHIR-99021 clinical trial pasta were determined using a colorimeter (BYK Gardner, Germany) with an illuminant D65 (daylight) and a visual angle of 10°. The ΔE values (i.e., the colour difference between two spectrophotometric measurements) were calculated according enough to Equation (2): equation(2) ΔE=(Lt∗−L0∗)2+(at∗−a0∗)2+(bt∗−b0∗)2where ‘t’ represents a specific storage period and ‘0’ is the beginning of storage. The reference sample was the pasta in the beginning of storage (t = 0). The sorbic acid content in the fresh pasta was assessed by ultraviolet absorption spectrometry (UV) at 530 nm AOAC 975.31 (1995). The results of mechanical properties, colour parameters, opacity and water vapour permeability were analysed using analysis of variance (ANOVA); treatment means were compared using Tukey’s test and Student’s t-test at a 5% significance level (p < 0.05) using a Statistica 8.0 software (Stat-Soft, Tulsa, OK, USA). The opacity of all films containing potassium sorbate (Fig. 1) was lower than that of the control films (CF), most likely due to the presence of sorbate, which acted as a plasticiser, thereby allowing a higher mobility between the starch molecules, and the passage of electromagnetic radiation. The FS4.5 film became more opaque during storage, most likely due to starch retrogradation.