monocytogenes infection,6,7,18,27,35 we compared the levels of IFN-γ primed by infection in IL-21-deficient and control mice (Fig. 2a). For both groups
of mice, the serum concentration of IFN-γ peaked sharply 24 hr post-infection, and although FK506 clinical trial there was a trend towards reduced levels in IL-21-deficient mice, these differences did not reach statistical significance. Thereafter, IFN-γ levels declined rapidly to baseline levels in both groups of mice. As IL-21 can directly stimulate and control IFN-γ production by NK and T cells,6,7,18 and IFN-γ production by these specific cell types has been directly implicated in innate L. monocytogenes host defence, the relative levels of IFN-γ produced by each cell type was also enumerated. The NKp46 marker that specifically identifies NK cells was used because IL-21 directly controls
the expression of other NK this website cell surrogate antigens (e.g. NK1.1).36,37 Interestingly, this analysis revealed no significant difference in percentage or absolute number of IFN-γ-producing NK and T cells 24 hr after infection, which corresponds to the peak serum concentration for this cytokine (Fig. 2b,c). Together, these results demonstrate that IL-21 plays a non-essential role in innate host defence and early IFN-γ production after L. monocytogenes infection. Given the importance of IL-21 in priming virus-specific CD8+ T cells in the adaptive immune phase,15–18 additional
experiments interrogated the requirement for IL-21 in the priming and expansion of L. monocytogenes-specific CD8+ T cells. Although IL-21, IL-12 and type I IFNs can each independently provide the ‘third signal’ for naive CD8+ T-cell expansion after stimulation in vitro, our recent studies also indicate that IL-12 and type I IFN receptor are simultaneously dispensable for the priming and expansion of L. monocytogenes-specific CD8+ T cells after infection in vivo.7,30,31,38 Accordingly, we hypothesized that IL-21 may mediate the IL-12-independent Epothilone B (EPO906, Patupilone) and type I IFN receptor-independent priming of L. monocytogenes-specific CD8+ T cells. To test this hypothesis, the expansion of L. monocytogenes-specific CD8+ T cells was enumerated for IL-21-deficient mice, and directly compared with the L. monocytogenes-specific CD8+ T-cell response in mice with combined defects in both IL-12 and type I IFN receptor (DKO mice), and mice with combined defects in IL-21, IL-12 and type I IFN receptor (TKO mice). For these experiments, the attenuated strain Lm-OVA ΔactA was used. The expression of the immune dominant H-2Kb OVA257–264 peptide antigen in this recombinant L. monocytogenes allows the antigen-specific CD8+ T-cell response to this surrogate L.
“Please cite this paper as: Bruns, Watanpour, Gebhard, Flechtenmacher, Galli, Schulze-Bergkamen, Zorn, Büchler and Schemmer (2011).
Glycine and Taurine Equally Prevent Fatty Livers from Kupffer Cell-Dependent Injury: An In Vivo Microscopy Study. Microcirculation 18(3), 205–213. Background: IRI still is a major problem in liver surgery due to warm ischemia and organ manipulation. Steatosis is not only induced by diabetes, hyperalimentation, alcohol and toxins, but also chemotherapy given before resection. Since steatotic livers are prone to Kupffer cell-dependent IRI, protection of steatotic livers is of special interest. This study was designed to compare the effect of taurine and glycine on IRI in steatotic
livers. Materials and Methods: Steatosis was induced with ethanol selleckchem (7 g/kg b.w.; p.o.) in female SD rats. Ten minutes after inactivation of Kupffer cells with taurine or glycine (300 mM; i.v.), left liver lobes underwent 60 minutes of warm ischemia. Controls received the same volume of valine (300 mM; i.v.) or normal saline. After reperfusion, white Panobinostat ic50 blood cell-endothelial interactions and latex-bead phagocytosis by Kupffer cells were investigated. Liver enzymes were measured to estimate injury. For statistical analysis, ANOVA and Student’s t-test were used. Results: Glycine and taurine significantly decreased leukocyte- and platelet-endothelium interactions and latex-bead phagocytosis
(p < 0.05). Liver enzymes were significantly lower after glycine and taurine (p < 0.05). Conclusions: This study shows that preconditioning with taurine or glycine is equally effective in preventing injury to fatty livers most likely via Kupffer cell-dependent mechanisms. "
“Angiogenesis is a multistep process that requires intricate changes in cell shape to generate new blood vessels. IF are a large family of proteins that play an important structural and functional role in forming and regulating the cytoskeleton. Vimentin, a major type III intermediate filament protein is expressed in endothelial and other mesenchymal cells. The structure of vimentin is conserved in mammals and shows dynamic expression profiles in various cell types and different developmental stages. Although initial studies with vimentin-deficient BCKDHA mice demonstrated a virtually normal phenotype, subsequent studies have revealed several defects in cell attachment, migration, signaling, neurite extension, and vascularization. Regulation of vimentin is highly complex and is driven by posttranslational modifications such as phosphorylation and cleavage by intracellular proteases. This review discusses various novel functions which are now known to be mediated by vimentin, summarizing structure, regulation and roles of vimentin in cell adhesion, migration, angiogenesis, neurite extension, and cancer.
Additionally, mRNA expression levels of pattern recognition receptors and immunomodulatory cytokines in the jejunum were investigated. T-cell receptor-γδ+ T cells were found to be increased in the gut mucosa 4 days after infection learn more and were most likely
involved in the primary local immune response. Five to eleven days later, cytotoxic T cells peaked in this location, which was preceded by an expansion of this lymphocyte population in the mesenteric lymph nodes. In intestines of infected piglets, mRNA expressions of TLR-2, NOD2 and TNF-α were significantly upregulated, suggesting an involvement in parasite recognition, immune response and possibly also in immunopathology. Taken together, this study identifies cellular and molecular players involved in the early immune responses against C. suis, but their precise role in the pathogenesis and control of this neonatal disease requires further investigation. “
“The immunological hallmark of Omenn syndrome (OS) is the expansion and activation of
an oligoclonal population of autoreactive T cells. These cells should be controlled rapidly by immunosuppressive agents, such as cyclosporin A (CsA), to avoid tissue infiltration and to improve the general outcome of the patients. Here we studied the clinical and the immune response to CsA in two Omenn patients and also examined the gene expression profile associated with good clinical response to such therapy. T cell receptor diversity was studied in cells obtained from OS patients selleck products during CsA therapy. Characterization of gene expression in these cells was carried out by using the TaqMan low-density array. One patient showed complete resolution of his symptoms after CsA therapy. The other patient showed selective response of his oligoclonal T cell population and combination therapy was required to control his symptoms.
Transcriptional profile associated with good clinical response to CsA therapy revealed significant changes in 26·6% of the tested genes when compared with the transcriptional profile of the cells before treatment. Different clinical response to CsA in two OS patients is correlated with their immunological Pyruvate dehydrogenase response. Varying clonal expansions in OS patients can cause autoimmune features and can respond differently to immunosuppressive therapy; therefore, additional treatment is sometimes indicated. CsA for OS patients causes regulation of genes that are involved closely with self-tolerance and autoimmunity. Omenn syndrome (OS) is an autosomal recessive severe combined immunodeficiency (SCID) characterized by generalized scaly exudative erythrodermia, enlarged lymph nodes, hepatosplenomegaly, severe susceptibility to infections, activation of T helper type 2 lymphocytes, eosinophilia and hyper-immunoglobulin (Ig)E .
We found that SOCS1 levels were raised in Adv-PTB as compared to the Mod-PTB group. This is the first report showing an increase in SOCS1 with more severe TB infections. Reduced mycobacterial antigen-specific IFN-γ levels have been reported in patients with far advanced TB , and previous studies have shown a decrease in M. tuberculosis-specific CD4 T-cell responses to be associated with cavitary disease . Our data suggest that increasing SOCS1 mRNA expression levels in patients with Adv-PTB may result in down-modulation of Th1-type responses, hence contributing selleck chemicals llc to the decreased mycobacterium-specific immunity observed in these patients. We observed that SOCS3 mRNA transcripts were
increased in T cells as compared with non-T cells in both TB and EC. However, we did not observe differences in the SOCS3 mRNA expression levels between TB and EC. Reports selleck screening library have shown SOCS3 expression to be increased in T cells of patients with active TB as compared with individuals with latent disease but not as compared with un-infected healthy control subjects . Therefore, our results are in concordance with previous data. Altogether, our study suggests that the expression of SOCS1 increases with the disease severity in TB. Upregulation of SOCS1 by M. tuberculosis
may be an effective strategy to counteract Th1-mediated IFN-γ responses and to increase disease pathology in the host. Thanks for help with patient recruitment to Dr. Nawal Salahuddin, Aga Khan University, Pakistan; to Muniba Islam for technical assistance; to Maqboola Dojki for administrative assistance. This study was supported by a SIDA Asia Link Program Grant, Swedish Research Council, and a University Research Council Grant, The Aga Khan University, Pakistan.
None declared. Conception Metalloexopeptidase and design: ZH and MR; Analysis and interpretation: ZH, MR, KI, MA, BC, RH, NR; Drafting the manuscript for important intellectual content: ZH, MR, KI, RH. “
“The altered expression of micro-RNA (miRNA) has been associated with Crohn’s disease (CD) and ulcerative colitis (UC). The aim of this study was to establish specific miRNA expression patterns in the serum and mucosa of inflammatory bowel disease (IBD) patients (UC and CD with colonic involvement) at different stages of the disease. Serum and biopsies from nine active CD (aCD), nine inactive CD (iCD), nine active UC (aUC) and nine inactive UC (iUC) and serum from 33 healthy subjects were collected. Up to 700 miRNAs were evaluated by the TaqMan® human miRNA array. The ΔCt values were obtained using the mean expression values of all expressed miRNAs in a given sample as a normalization factor for miRNA real-time quantitative polymerase chain reaction data. The levels of serum miRNAs in CD and UC patients were different to healthy subjects. Thirteen serum miRNAs were expressed commonly in CD and UC patients.
5% versus 0%, P = 0.001). Body weight did not change significantly in the icodextrin group, but body weight in the control group increased from 63.3 ± 14.5 kg at baseline to 64.2 ± 14.2 kg
at day 5 (P = 0.0002) and 65.2 ± 14.1 kg at day 10 (P < 0.0001). Conclusion: As compared with glucose-based peritoneal dialysis solution, use of icodextrin achieved better ultrafiltration and fluid control during acute peritonitis complicating continuous ambulatory peritoneal dialysis, although we found no evidence of a worthwhile clinical benefit on peritonitis resolution. (ClinicalTrial.gov number, NCT0104446 [ClinicalTrial.gov].) SUGIURA TOSHIHIRO1, AKAGAKI FUYUKO1, KUBOTA KEIICH1, NAKAMORI AYA1, WADA AKIRA2 1Otemae BMN 673 research buy Hospital, Japan; 2Osaka National Hospital, Japan Introduction: Recent studies have shown that renal resistive index (RI) reflects systemic vascular stiffness as well as renal arteriolosclerosis. While this fact makes it difficult to interpret the increase in RI, we have shown that high RI is an independent risk factor for worsening renal function and can estimate renal prognosis in CKD [Nephrol Dial Transplant 2009; 24: 2780–5, Clin Exp Nephrol 2011; 15: 114–20]. The purpose of the present study is to determine the relative risks with an increase in RI for progression of CKD. Methods: We
performed a 2-year follow-up study with an observational cohort of 429 CKD patients (GFR 45 ± 31 mL/min/1.73 m2, age 57 ± 17 years). The patients were examined by Doppler ultrasonography for RI [(peak-systolic velocity – end-diastolic hypoxia-inducible factor cancer velocity) / peak-systolic velocity] to be calculated. Glomerular filtration rate (GFR; mL/min/1.73 m2) was estimated from serum creatinine (s-Cr) and age with the revised Japanese equation: 194 × s-Cr−1.094 × Age−0.287 (×0.739 for women).
Worsening renal function was defined as a decrease in GFR of at least 20 mL/min1.73 m2 or the need for long-term dialysis therapy until the end of the 2-year follow-up. Results: Among the 429 CKD patients, 107 patients presented with worsening renal function during the 2-year follow-up. When we divided the patients into cAMP three groups by RI value of 0.70 and 0.80, Kaplan-Meier analysis showed that the event-free survival rates of worsening renal function at 24 months were 0.93, 0.70 and 0.35 in patients with RI ≤ 0.70, 0.7 < RI ≤ 0.80 and RI > 0.80 respectively (Log-rank test, P < 0.001, Fig. 1). Cox proportional-hazard analysis showed that the adjusted hazard ratio (HR) for worsening renal function was 4.54 [95% confidence interval (CI) 2.31–8.96, P < 0.001] and 2.81 [95% CI 1.48–5.35, P < 0.01] in patients with RI > 0.80 and 0.7 < RI ≤ 0.80 respectively, as compared with the patients with RI ≤ 0.70. HR was adjusted by the factors that could influence RI itself and/or renal outcome, namely, age, GFR, urinary protein excretion, systolic blood pressure, and use of renin-angiotensin system (RAS) inhibitors.
Moreover, a novel subpopulation of human MDSC has recently been described possessing strong T-cell suppressive potential. This subset was induced from normal peripheral blood mononuclear cells using cytokine mixtures containing IL-1β 35. Ly6Cneg-MDSC and Ly6Clow-MDSC might represent separate lineages of MDSC characterized by a different susceptibility to factors in the tumor/host environment and equipped with a differential capacity to interfere with adaptive and innate immune responses. Alternatively,
variations in the level of expression by PMN-MDSC of Ly6C might mark distinct states of differentiation within one MDSC lineage. Conceivably, such a differentiation within the tumor-microenvironment would likely be susceptible AZD0530 clinical trial to tumor-derived signals, including tumor-derived factors. In support of this, it has recently been shown that different tumor microenvironments harbor distinct subsets of AZD2281 manufacturer tumor-associated macrophages that could be classified according to the “M1” (antitumor) versus “M2” (protumor) macrophage activation paradigm 36 and all of which could be derived from a common monocyte
precursor population 36. A similar plasticity has been reported to exist within tumor-associated neutrophils that could polarize under the influence of TGF-β present in the tumor-microenvironment toward antitumorigenic “N1” (when blocking TGF-β) versus protumorigenic “N2” (presence of TGF-β) subsets 37, 38. Whether or not Ly6Cneg-MDSC can be classified according to this paradigm requires further experimental investigation. NK cells are generally described as prototypic innate anti-tumor cells 27, 28 and an impaired NK cell compartment is associated with enhanced susceptibility to tumor development 39–41. Consequently, a coherent “survival” strategy
of tumors might involve impairing the activity of NK cells, which is indeed frequently observed in tumor-bearing individuals 18, 26–29, 42, 43. The block in the development of NK cells from 4T1/IL-1β-tumor-bearing mice is similar to that observed in mice bearing EL4 tumors 44 and reminiscent of NK cells from transgenic mice expressing the CD27-ligand CD70 ectopically on all B cells 45. The reduced level of CD27 expression by NK Clomifene cells might thus be an indication of engagement of CD27 by its ligand CD70, suggesting that constitutive CD27-CD70 interactions might cause the observed block in NK cell development in 4T1/IL-1β-tumor-bearing mice. As CD70 expression is restricted to activated T and B cells, its expression might be induced upon exposure to IL-1β. However, NK cells in CD70-tg mice were not functionally impaired and expressed high levels of NKG2D, suggesting that the functional inhibition of NK cells in 4T1/IL-1β-tumor-bearing mice is independent of the developmental defect. Suppression of NK cell function in tumor-bearing mice has been shown to involve MDSC-derived cytokines including TGF-β1 18.
4%), helpful in learning (84.2%), better than traditional MMC (94.7%). Conclusion: A structured MMC is an effective means of engaging physicians, nurses, and key administrative leaders in the discussion of adverse events or patient complications. This systems-based, problem-learning process can promote patient care and safety. RAFIQ KAZI1, U0126 concentration SHERAJEE
SHAMSHAD J.1, FUJISAWA YOSHIHIDE2, MOGI MASAKI3, RAHMAN ASADUR1, SUFIUN ABU1, NAKANO DAISUKE1, KOEPSELL HERMANN4, NISHIYAMA AKIRA1 1Department of Pharmacology, Faculty of Medicine, Kagawa University; 2Life Science Research Center, Faculty of Medicine, Kagawa University, Japan; 3Department of Molecular Cardiovascular Biology and Pharmacology, Graduate School of Medicine, Ehime University, Japan; 4Institute of Anatomy and Cell Biology, University of Wuerzburg, Germany Introduction: Overactivity of the sympathetic nervous system has been shown as one of the major contributors to the complex pathophysiology of hypertension, hyperinsulinemia and diabetes. Renal sympathetic denervation (RDX) improves glucose metabolism and insulin sensitivity in addition to reducing blood pressure in patients with resistant hypertension. We investigated the effects of renal sympathetic AZD2014 chemical structure denervation at early age on the development of hypertension
and glucose metabolism in obese rats. Methods and Results: Uninephrectomized (at 5 week of age) Otsuka Long Evans Tokushima Fatty (OLETF)
and Long Evans Tokushima Otsuka (LETO) were underwent RDX at 6 week of age. RDX-LETO and -OLETF rats had almost undetectable Leukocyte receptor tyrosine kinase kidney tissues norepinephrine (NE) levels. RDX did not affect blood pressure profiles and heart rate in pre-diabetic stage evaluated by telemetry system. RDX-OLETF rats showed markedly lowered in the blood glucose, plasma insulin levels and their area under the curve in response to oral glucose loading during the oral glucose tolerance test compared to non-denervated OLETF rats. Furthermore, the whole body insulin sensitivity was assessed by the hyperinsulinemic-euglycemic clamp study at 20 week of age, and RDX-OLETF rats showed an increased glucose infusion rate than non-denervated OLETF rats. RDX suppressed plasma and renal tissues NE levels and increased in vivo glucose uptake by adipose tissues, soleus muscle and liver tissues in OLEFT rats. Furthermore, RDX suppressed sodium dependent glucose transporter 2 (SGLT2) translocation and expression in renal proximal tubular brush border membrane as detected by immunofluorescence and western blot followed by markedly increased urinary glucose excretion in OLETF rats.
77,78 Mechanistically, the effect of IL-17E on disease is linked to expression of IL-23 and IL-13. In the absence of IL-17E signals, IL-23, a critical mediator of Th17 cell survival and maintenance, is elevated, whereas the reduction in disease severity seen with IL-17E treatment is linked to increased expression of IL-13, which in turn blocks IL-23 secretion by dendritic cells, preventing Th17 cell survival.77,78 Similarly, IL-17E inhibited Th1 cell-driven colitis through blockade of IL-12 and IL-23 expression by CD14+
cells isolated from the inflamed gut of patients with IBD.79 These studies together with the observation Smoothened antagonist that IL-17E expression is down-regulated in the inflamed colon tissue of patients with Crohn’s disease or ulcerative colitis, suggest the possible use of IL-17E as a therapeutic agent for IBD.79
The cellular source(s), receptor utilization and target cells of the IL-17B, IL-17C and IL-17D family members are poorly characterized. Initially discovered using database searches for homology to IL-17A, it is unclear whether these cytokines share similar biological properties (Fig. 1).80–82 Based on sequence comparison to IL-17A it is hypothesized that these family PS-341 members also form dimers, although biochemical analysis of IL-17B suggests that it forms a tightly associated, non-disulphide linked dimer, which is in contrast to what is observed PRKD3 with IL-17A and IL-17F.82 How IL-17C and IL-17D behave is undetermined. Although a specific high-affinity interaction was observed between IL-17B and the IL-17RB subunit using in vitro biochemical assays, the import of this finding is unclear.82 Likewise, while IL-17C has been reported to associate with IL-17RE, the functional significance of this interaction has not been demonstrated.7 The receptors for IL-17D are unknown. Expression profiling has provided some information on the cellular sources of these cytokines (Table 1). Expression
of IL-17B protein has only been reported in neurons and chondrocytes.81–86 Interleukin-1β treatment of bovine cartilage explants promoted secretion of IL-17B,87 suggesting that expression is modulated by pro-inflammatory stimuli. Similarly, although basal IL-17C mRNA is undetectable, significant induction is observed after exposure to inflammatory signals.81 Tumour necrosis factor-α stimulated IL-17C secretion from human keratinocytes, whereas the TLR5 agonist, flagellin, promoted il17c mRNA expression in murine colon tissues.9,88 Details of IL-17D protein expression have been reported.80 Pre-clinical and clinical studies suggest that expression of these family members is modulated by inflammation. Both IL-17B and IL-17C were detected in the paws of mice afflicted with collagen-induced arthritis, with IL-17B exclusively found in chondrocytes while IL-17C was detected in several populations of leucocytes.
pylori infection and the presence of pernicious anaemia are the leading contenders. In 2008 strategies for preventing gastric cancer
were reviewed Angiogenesis inhibitor systematically at the Asia-Pacific Gastric Cancer Consensus Conference . It was concluded that H. pylori screening and eradication in high-risk populations reduced the relative risk of gastric cancer (RR 0·56, 95% CI 0·4–0·8) [44,49]. Other studies have shown that eradication therapy promotes regression and prevents the progression of some precancerous gastric lesions [49,50]. Diagnosis of H. pylori infection cannot be made by serology in CVID patients, but depends on a urea breath test (UBT), faecal antigen immunoassays or endoscopic biopsy. The UBT is the gold standard test. It is widely available, non-invasive, cheap, sensitive (90·3%; 95% CI 83–95) and specific (89·5%; 95% CI 81–95) , making it the most suitable for detecting H. pylori infection in CVIDs . The stool test is equally sensitive (sensitivity 68·8–91·7%; specificity 75·6–88·9%) and there is little significant difference in the cost. However, 60% of patients prefer the UBT to the stool test . Because infection is often asymptomatic, detection and eradication of H. pylori at an early stage is appealing. Once eradicated, H. pylori almost
never recurs in the general adult population , although it is unknown whether this also applies to patients with CVIDs buy MI-503 who lack protective immunoglobulin (Ig)A at mucosal surfaces. Diagnosis of pernicious anaemia is detected by measuring iron and serum B12 as screening tests for gastritis and vitamin deficiency. Consequently, three simple, non-invasive tests (UBT, serum iron and serum B12) are likely to identify patients with CVIDs who are at the highest risk of gastric cancer in a screening protocol. Regardless of the presence of pernicious anaemia or H. pylori infection, patients with CVIDs still have a 10-fold increased risk  for gastric cancer, so can reasonably be regarded as a high-risk population.
Although endoscopic screening of all patients with CVIDs could be considered, a more selective approach is appropriate. We propose (Fig. 1) that all patients diagnosed with CVIDs diglyceride should undergo screening for H. pylori, using the UBT, at diagnosis. If positive, H. pylori eradication should follow standard practice, with a repeat breath test to demonstrate effective treatment. Because recurrence of infection is exceptional in developed countries  a breath test at diagnosis is likely to be sufficient, although data to support this in CVID patients are lacking. In addition, all patients should have serum B12 and iron concentrations measured annually, as pernicious anaemia or gastritis may develop at any age.
Palliative care services in conjunction with the primary care and renal teams should play a role in educating community members in how they can support the person and the family, thus helping to meet the person’s choice of place to ‘finish up’ and helping family/community members feel they have appropriately supported the patient in the ‘finishing up’ process. As recommended by the American Society of Nephrology, Galla, there is a clear need to strengthen partnerships between palliative care and renal services if the best care and support is to be provided for a person opting for the non-dialysis pathway. Choice of place of death: being able to ‘finish up’ in the place of
their choice is very important to many indigenous Australians, with strong connections to traditional lands playing an important cultural role. However cultural practices INCB024360 supplier and requirements may vary from
community to community, and even within communities (particularly in urban areas). If a patient wishes to stay on or return to their homeland to die, these arrangements will need to Acalabrutinib be planned and supported. The effectiveness of renal supportive care may also strongly correlate with issues such as: person not being able to fully understand their illness; difficulties in communication and the length of time it takes to gain a person’s trust. Each indigenous person is different and therefore should not be stereotyped. One should not make assumptions of ATSI people and remember that each case is considered on an individual basis, without prejudice or judgement. Establish a commitment to the patient, build trust and be consistent. Respect ATSI cultural protocols, practices and customs. Respect ATSI decision-making processes. For most indigenous people having the family involved is extremely important. Families, Carnitine palmitoyltransferase II as mentioned above can include an extensive range of relatives. However there are individual variations.
Institutions such as hospitals and dialysis units, nursing homes must take responsibility for facilitating culturally competent care. This includes knowing the groups that most frequently use the institution, seeking out and disseminating information about cultural beliefs that might affect attitudes towards illness and health care, providing adequate translation services, and identifying community resources. Hiring and training health care workers (at all levels) who are members of the ethnic group in question or knowledgeable about them and who have credibility within these communities may assist greatly in bridging the cultural chasm. Health professionals need to acknowledge the beliefs and practices of people who differ from them in age, occupation or social class, ethnic background, sex, sexuality, religious belief and disability.