“
“Background and Purpose: Laparoscopic donor nephrectomy has become the procedure of choice for living kidney transplantation in many centers. We report on our experience with laparoscopic hand-assisted Savolitinib donor nephrectomy, in particular concerning graft function compared with open donor nephrectomy. Materials and Methods: Between 1995 and March 2007, 72 patients with end-stage renal disease have received kidney transplantation from living donors. Open living donor nephrectomy
(ODN) was performed in 35 donors, whereas 37 donors had undergone laparoscopic hand-assisted nephrectomy (HALDN). Immediate graft function, serum creatinine and serum cystatin C 1 year after the transplantation were evaluated. Results: Median operative time was 138 min (113-180 min) in the HALDN group and 112 min (91-162 min) in the ODN group (p < 0.05). Warm ischemia time was 87 s (63-150 s) in the HALDN and 81 s (56-123 s) in the ODN groups, respectively (p = 0.13). Both the rate of primary graft function as well as kidney graft function parameters serum creatinine and serum cystatin C 1 year after transplantation showed no statistically significant difference
between the two groups of patients. Conclusions: Laparoscopic Stem Cell Compound Library price hand-assisted donor nephrectomy is safe and has no negative impact on the transplanted graft function when compared with open donor nephrectomy. Copyright (C) 2010 S. Karger AG, Basel”
“Purpose: To assess the potential of using the residual phosphorylation of histone H2AX (gamma H2AX) after irradiation as a marker of radiosensitivity in vitro.
Material and methods: Confluent cell cultures of FaDu and SKX human squamous cell carcinoma lines were irradiated with ZIETDFMK graded single doses. Twenty-four hours after irradiation cells were seeded for standard colony forming assay (CFA). In parallel, staining for gamma H2AX was performed to visualise the residual foci.
Results: In the CFA, FaDu showed a higher radioresistance
than SKX. After analysis of the residual foci data, we constructed ‘predicted’ survival curves using two different methods. First, the proportion of nuclei with <3 foci was found to correlate closely with the observed surviving fraction (SF) in FaDu, with a slight overestimation of the true SF in SKX. Second, there was a strong linear correlation of the mean number of residual foci and observed -lnSF. Based on regression analysis, we calculated the SF for both cell lines based on the mean number of residual gamma H2AX foci. This second approach again led to a good correlation of predicted and observed SF values in FaDu and a (slight) overestimation in SKX.
Conclusion: In the two cell lines investigated the mean number of residual foci of gamma H2AX can be used to predict differences in the radiation dose response relationship in vitro.”
“Objectives: We analyzed whether the American Society of Anesthesiology (ASA) classification could be used as a prognostic factor in renal cell carcinoma.