1). Samples with large fibers absorbed TCH less efficiently than those with small fibers because they had less specific surface area to absorb TCH. As a consequence, samples D, Wortmannin cost P and Ca absorbed and released less TCH than the control (p < 0.05). Samples D, P and Ca absorbed and released similar amounts of TCH during the course of the test (p > 0.05). It seemed that the TCH release percentages of the samples corresponded with their swelling ratios. Samples P and Ca had the highest and lowest swelling ratios of all and they also had the highest and lowest parentages of TCH release, respectively, at 6 h. Samples with more loose fibrous structures swelled up faster (section 2.3) and as a consequence released the absorbed TCH more quickly. Figure 6. The cumulative TCH release of different samples over 6 h in PBS.

Data are presented as the mean �� SD (n = 6). The total amount of TCH released within 48 h was the amount of TCH loaded on the sample. The percentages of TCH released … Alginate itself and what it might release did not inhibit the growth of E. coli (Fig. 7A). Only the TCH released from the samples did inhibit the growth of E. coli and form an inhibition zone. Sample A had the largest inhibition zone of all of the samples (p < 0.05) (Fig. 7B), most likely due to the highest amount of TCH it released. Sample P had a larger inhibition zone than the control and Ca had a smaller one (both p < 0.05). The inhibition zone of sample D was similar to that of the control (p > 0.05). There did not seem to be a strong correlation between the amount of TCH loaded, their release pattern, and the size of inhibition zone.

However, the most important issue in wound care is not the delivery mechanism but rather finding medications that can be topically absorbed, which provides desirable therapeutic effects within wounds.4 Figure 7. (A) Representative pictures of the antibacterial effect of samples releasing tetracycline HCl (TCH). (B) The bacterial inhibition zone of the samples loaded with (TCH). Data are presented as the mean �� SD (n = 8). *Indicates … Materials and Methods Sample preparation Alginic acid monosodium salt was extracted from brown seaweed with a ratio of mannuronic acid to guluronic acid (M/G ratio) of 1.56 and a molecular weight of 100,000 Da (Sigma). The viscosity of the 2% alginate aqueous solution at 25��C was 150 cP (ARES Rheometer, Rheometric Scientific).

Alginic acid monosodium salt was dissolved in deionized water, and the solution was loaded into a plastic syringe, which was immobilized onto a robotic arm and kept at room temperature. Air pressure was applied to the syringe through a pneumatic regulator (8000D, Ganbow) to expel the alginate solution through a needle into a container filled with calcium chloride solution in which the gelation process Cilengitide of the alginate fibers occurred. For the tensile and water vapor transmission rate tests, 35 mL of alginate solution was extruded into a plastic container (5.5 cm �� 3.