“
“Aims:
To develop a scanning electron microscopic approach using in situ hybridization (SEM-ISH) for gaining both genetic and morphological information about target bacteria.
Methods and Results:
Target cells were hybridized with DNA-targeted polynucleotide probes, and a tyramide signal amplification system was used to increase the sensitivity. The protocol of SEM-ISH enabled to detect low copy number target DNA sequences in individual cells.
Conclusions:
SEM-ISH allowed the in situ detection of bacteria https://www.selleckchem.com/products/selonsertib-gs-4997.html carrying a specific gene.
Significance and Impact of the Study:
Combining morphological study with SEM and ISH techniques appears to be a valuable tool to understand
the spatial distribution of target cells in complex microbial communities on various materials.”
“Aims:
Oxalobacter formigenes is an oxalate-degrading click here intestinal bacterium that has been found in humans, cattle, sheep, rats and dogs.
Its presence in the intestinal tract may be a protective factor against calcium oxalate urolithiasis because of its ability to degrade oxalate. The objective of this study was to determine whether O. formigenes could be detected in the faeces of healthy cats.
Methods and Results:
A convenience sample of 28 cats was enrolled. Faecal samples were tested for oxc, a gene specific for O. formigenes, by real-time PCR. This gene was detected in 5/28 (18%) cats; however, the prevalence increased to 86% (24/28) with a modification of the methodology.
Conclusions:
Demonstrating the presence of O. formigenes in the faeces of healthy cats for the first time in this study.
Significance and Impact of the Study:
Future investigation of the role of this organism in the pathophysiology of calcium oxalate urolithiasis in cats is indicated.”
“Aims:
To investigate bacterial contamination on hand-touch surfaces in the public transport system and in public areas of a hospital in central London.
Methods
and Results:
Dipslides were used to sample 118 hand-touch surfaces in buses, trains, stations, hotels and public areas of a hospital in central MLN2238 clinical trial London. Total aerobic counts were determined, and Staphylococcus aureus isolates were identified and characterized. Bacteria were cultured from 112 (95%) of sites at a median concentration of 12 CFU cm-2. Methicillin-susceptible Staph. aureus (MSSA) was cultured from nine (8%) of sites; no sites grew methicillin-resistant Staph. aureus (MRSA).
Conclusions:
Hand-touch sites in London are frequently contaminated with bacteria and can harbour MSSA, but none of the sites tested were contaminated with MRSA.
Significance and Impact of the Study:
Hand-touch sites can become contaminated with staphylococci and may be fomites for the transmission of bacteria between humans.