Addition of telomeric repeats to the DNA strands nge r. Use TER TERT Restrict Nken k Can and to protect the ends of chromosomes by the addition of a sequence of six nucleotides repeated 5 TTAGGG 3 stranded chromosomes. The expression CI-1033 of hTERT is the limiting factor of the human telomerase activity determinant t and it is assumed that a sensitive indicator of the function of the telomerase activity t be. However, remain the means by which regulated TA largely unknown. TA was observed in 85% of all human tumors, suggesting that the immortality conferred by telomerase, an r Key plays in the malignant transformation. TA has been shown in bone marrow cells of CML patients w During the progression of the disease to increased hen.
Transfection of the catalytic subunit of telomerase, erm Glicht hTERT cultured primary Ren human cells with SV40 large T antigen and N ras oncogene-transformed cells to overcome the crisis and ultimately reach a b Sartigen tumor. This suggests that telomerase upregulation can actively contribute to cellular Ren immortalization and tumorigenesis in human cells. Therefore, Clinofibrate telomerase is considered as an attractive target for cancer diagnosis and cancer therapy. TA and the expression of telomerase components confinement on several levels, Lich transcription and transcription in accordance with pr Regulated precise assembly and the correct position. TA may also regulated at post-translational studies have shown that the protein kinase C and upregulate AKT / protein kinase B k Can human TA by phosphorylation of hTERT. Several studies have reported that Glivec can regulate TA.
However, the mechanism, not with Gleevec TA button cells in BCR ABL is clear. Conflicting results from different studies have been obtained, some have shown that Gleevec treatment, the L Length of telomeres and TA increased to Hen, w While a recent study showed that Gleevec TA reduced in K562 cells, BCR ABL positive. because the BCR-ABL is the specific target of Gleevec, we assumed that Gleevec TA button. by regulating the expression and activation of telomerase by BCR-ABL In this study we investigated the effect of Glivec on the online technical support BCR-ABL positive cells and cell lines deficient. Our results show that the treatment Glivec significantly inhibits the technical and reduces the expression of hTERT mRNA level in K562 cells but not in Jurkat and HL60 cells.
Zus Tzlich slaughter STAT5A siRNA resulted in a marked down-regulation of hTERT mRNA, protein and TA in K562 cells. We have also found there K562 cells, a significant increase Erh Tyrosine phosphorylation in the hTERT that w Was reduced during the treatment Gleevec in K562 cells but absent to show in HL60 cells. In addition, we have also seen the release of treated hTERT nucleolar nucleoplasm of Gleevec K562 cells. These results emphasize the r Potential of BCR-ABL in the regulation of telomerase and imply that BCR ABL, the expression of telomerase activity of t Regulated at the transcriptional level by Jakstat and post-translational level by phosphorylation. K562 cell culture methods, KU812, HL60 and Jurkat cell lines were obtained from the American Type Culture Collection in RPMI supplemented with 10% heat inactivated FBS, 100 units / ml penicillin, 100 g / ml streptomycin and 2 mM L glutamine at 37 in a incubator with 5% CO2. Ap