citrinum and related species are examined using the ITS regions (intergenic spacer region and 5.8S rDNA gene) and parts of the β-tubulin and calmodulin gene, in combination with extrolite profiles, physiology and macro- and microscopical characters. A large set of isolates, including the type strains of various synonyms and freshly isolated strains are included in this study. Material
and methods Isolates The examined strains included type strains or representatives of species related learn more to P. citrinum. Additional strains were isolated from various substrates, such as soils from different locations, food- and feedstuffs and air. An overview of strains used in this study is presented in Table 1. All strains are maintained in the CBS culture collection. Table 1 Details of isolates included in the morphological and/or molecular examination of this study Species CBS number Substrate and locality P. citrinum 139.45 Ex type of P. citrinum and P. aurifluum, unrecorded source P. VRT752271 mw citrinum 252.55 Ex-type of P.botryosum, herbarium specimen, Recife, Brazil P. citrinum 241.85 IMI 092267; ex type of P. phaeojanthinellum, unrecorded source P. citrinum 122726 NRRL 783; representative of P. sartoryi, unrecorded source P. citrinum 115992 Compost, the Netherlands P. citrinum 122398 Peanut, Indonesia
P. citrinum 122397 Soil, Treasure Island, Florida, USA P. citrinum 865.97 Patient with acute myeloid leukemia, Hong Kong, China P. citrinum 122395 Coconut milk; produced in Indonesia, imported into the Netherlands P. citrinum 122394 Soil, Merang, Malaysia P. citrinum 232.38 Type of P. implicatum; original culture deposited
by Thom (as Thom 4733.73), unknown source, Belgium P. citrinum 117.64 Epoxy softener, the Netherlands P. citrinum 122452 Coffee beans, Thailand; colour mutant P. citrinum 122451 NRRL 2145; colour mutant;unrecorded source P. citrinum 101275 Leaf, Panama P. gorlenkoanum 408.69 Ex-type strain of P. gorlenkoanum; soil, Syria P. gorlenkoanum 411.69 Ex-type strain of P. damascenum; soil, Immune system Syria P. hetheringtonii 122392 Type; soil, Treasure Island, Florida, USA P. hetheringtonii 124286 Soil, Lookout Kuranda, Queensland, Australia P. hetheringtonii DTO 30H7 Soil, Lookout Kuranda, Queensland, Australia P. hetheringtonii 124287 Soil, Lake Easchem, Queensland, Australia P. sizovae 413.69 Neotype of P. sizovae; soil, Syria P. sizovae 122387 Margarine, the Netherlands P. sizovae 139.65 Sea salt, Portugal P. sizovae 122386 Glue, the Netherlands P. sizovae 115968 Cropped soil, Italy P. sizovae 117183 Papaver somniferum, the Netherlands P. sizovae 117184 IBT 22812; salty water in saltern, Slovenia P. steckii 325.59 Ex-type of P. corylophiloides nom. inval.;ex soil Japan P. steckii 789.70 Unrecorded source P. steckii 122391 Potting soil, the Netherlands P. steckii 260.55 Ex-neotype of P. steckii; cotton fabric treated with copper naphthenate, Panama P.