Concentrations of oxidants used were based on the amounts necessary to eradicate CFU viability as assessed in the previous experiments. A) All organisms displayed significant AZD1480 reduction in ATP production (One-way ANOVA) in an H2O2 dose-dependent manner up to 5 mM. B) ATP production by KP was statistically unaffected by HOCl exposure up to 0.1 mM according to one-way ANOVA (p = 0.53) while all other organisms tested displayed significant HOCl dose-dependent reduction in ATP production in this concentration range.

Error bars represent standard deviation of at least n = 3 experiments. Figure 6 Correlating H 2 O 2 -induced loss of ATP production with MK5108 cost bacterial viability. H2O2-induced disruption of ATP production correlated statistically with abolishment of CFU viability for all organisms tested except PsA (p = 0.15) at concentrations up to 5 mM. Though the decline of ATP production in PsA for this oxidant was statistically significant

in this range, the percent BKM120 change remains independent of the percent reduction in CFU viability. Solid circles and lines: ATP recovery after oxidant exposure. Open circles and dotted lines: CFU viability. Both parameters are measured as percent relative to oxidant-free controls. P-values represent linear regression of the raw data values from percent ATP recovery versus CFU viability. Values less than 0.05 were considered significant and denote correlation between the parameters; values greater than 0.05 indicate independence of the parameters. Error bars represent standard deviation of at least n = 3 experiments. ATP production was dose-dependently abolished in PsA, SA, BC, and EC while KP remained statistically unaffected even at HOCl doses up to 0.1 mM (PsA, p < 0.0001; SA, p < 0.0001; BC, p < 0.0001; EC, p < 0.0001 and KP, p = 0.53; Figure 5B). The decline in ATP production correlated with HOCl-induced loss of CFU viability in PsA, BC, and EC (p = 0.005, 0.006, and 0.01, respectively, Figure 7) but was independent

of diminished CFU viability in SA and KP (p = 0.20 and 0.60, respectively). Figure 7 Correlating HOCl-induced ATP changes with bacterial viability. ATP production is affected by HOCl exposure and correlates statistically with CFU viability in PsA, BC, and EC (p = clonidine 0.005, 0.006, and 0.01, respectively); however, SA and KP lose CFU viability after exposure to lower concentrations of HOCl than are required to abolish ATP production during the assay time. Solid circles and lines: ATP recovery after oxidant exposure. Open circles and dotted lines: CFU viability. Both parameters are measured as percent relative to oxidant-free controls. P-values represent linear regression of the raw data values from percent ATP recovery versus CFU viability. Values less than 0.05 were considered significant and denote correlation among the parameters; values greater than 0.05 indicate independence of the parameters. Error bars represent standard deviation of at least n = 3 experiments.