Freundons, that comprising mammary homogenate and Freund,s complete adjuvant evoked most foci of leucocytes in the mammae. GSK690693 Akt inhibitor Other combinations of saline, hepatic or mammary homogenates emulsified with Freund,s complete or incomplete adjuvants did not yield significantly different proportions of mammae containing 7 or more foci to that observed after oestrogenization alone. No lesion in other organs appeared attributable specifically to inoculation of mammary homogenate and Freund,s complete adjuvant. Such findings resemble those in other experimental organ specific lesions induced in the brain, testis, and thyroid. The low incidence and mildness of the arthropathies in all groups may be related to oestrogenization. However, the interaction between oestrogenization and the leucocytic reactions to inoculation was not tested here, for all inoculated animals were also oestrogenized.
The chronological development and morphology of the mammary lesions are also similar to many experimental allergic organ specific diseases. The interval of 14 days between inoculation and the appearance of the first leucocytic exudates, the gradually increasing DNA-PK Inhibitors size of the foci with parenchymal disruption, and the subsequent resolution of the lesions, which thus lack progressive character, resemble other experimental allergic models. The rat has shown a pronounced regression of experimental allergic lesions of the thyroid after the fourth and fifth weeks but a similar, if later, regression was also noted in the thyroid lesions of guinea pigs.
Though the mammary lesions remained strictly focal in oestrogenized animals, the microscopic morphology also resembles other models of experimental organspecific allergic disease. Mononuclear predominance in the inflammatory exudate. which has been stressed by Waksman as a hall mark of experimental autoallergic diseases, and focal destructive disruption of the parenchyma are consistent with cell mediated immunological reactions to elements of the mammary parenchyma. The augmentation of experimental autoallergic lesions by pertussis vaccine was not convincingly reproducible here. Perhaps the variation in effectiveness of different pertussis vaccines noted by Paterson et al. was responsible. The potency of the antilymphocyte serum was shown by the differential reduction of the peripheral blood lymphoid cell count by about 5000 and by the suppression of the paracortical nodules in the intramammary lymph nodes.
Though it apparently evoked an antibody response, as was shown by the plasma cell rich expansion of the medullary cords of the lymph nodes and the appearance of a precipitating antibody to rabbit serum proteins in the sera of the treated rats, the stability of the red blood cell and granulocyte counts in the treated animals would indicate that it was not toxic. These effects and the suppression of the mononuclear infiltrates in the mammae of rats inoculated with mammary homogenate and Freund,s complete adjuvant resemble the effects of antilymphocyte serum in experimental allergic thyroiditis in the rat. The appearance of granulocytes around mammarv lobules in the mammae in some of the animals administered antilymphocyte serum raises the possibility of a local Arthus type reaction in the mammae to xenogeneic immunoglobulin, which is