hydrophila. They were tested for protease activity and for inhibition of the antimicrobial activity of natural peptide mixtures and single peptides of all three frog species against Escherichia coli ATCC 25922. ECPs from cultures of Aer. hydrophila grown for 16, 24 and 36 h showed protease activity and inhibited the antibacterial activity of all peptides against E. coli ATCC 25922. In contrast, the ECPs from cultures of Kl. pneumoniae neither
had protease activity MLN2238 cost nor inhibited the activity of any peptides.
Conclusion:
The proteolytic ECPs of Aer. hydrophila have the ability to inhibit the skin antimicrobial peptides of frogs.
Significance and Impact of the Study:
The results of this study provide new information on the association of ECPs with the resistance of Aer. hydrophila to frog antimicrobial peptides.”
“Aims:
The genetic diversity of Campylobacter isolated from human infection and from poultry Danusertib ic50 was assessed in strains originating in three different European regions in order to compare these two hosts and to investigate European regional differences.
Methods and Results:
Randomly chosen isolates originated from Norway, Iceland and Basque Country in Spain were genotyped by sequencing of the short variable region (SVR) of flaA.
A total of 293 strains were investigated, c. 100 per country with half originated from either host. The results indicate extensive diversity in both hosts and identified differences in the nature and distribution of genotypes between the countries. These differences could in part be related to geographical location, in that Campylobacter genotypes from Iceland and Norway were more similar to each other than either was to Basque Country.
Conclusions:
Differences between the countries exceeded the observed differences between human
and poultry isolates within a country.
Significance and Impact of the Study:
Regional differences are extensive and should not be ignored when comparing genotyping data originating from different international studies.”
“Aims:
To isolate cultivable thermophilic lactic acid bacteria from cheeses made with selleck chemicals llc mesophilic starter and compare them with dairy-related Lactobacillus helveticus strains using molecular typing methods.
Methods and Results:
The number of thermophilic bacteria in seven commercial cheeses manufactured with mesophilic starters was estimated to be < 10 CFU g(-1). Implementation of an enumeration step in the isolation method made it possible to isolate one thermophilic strain from each of five of seven cheeses. Comparing repetitive sequence PCR (rep-PCR) profiles of the isolates with dairy-related Lact. helveticus strains indicated that one isolate was a Lact. helveticus. Partial sequencing of 16S rRNA confirmed this, and the remaining four strains were identified as Lactobacillus delbrueckii, Lactobacillus fermentum and Enterococcus faecium. The rep-PCR profile of the isolated Lact.