In contrast to a lack of tumor formation in controls, 62% of Ptc1/ mice and 67% of Gli31/ mice developed medulloblastomas. Our information indicate that mis expression of Shh alone within the postnatal brain will not be adequate to induce tumors effi ciently. Nevertheless, by also getting rid of one copy of Ptc or Gli3, medulloblastomas form at a appreciably increased rate. This research represents the primary evidence that Gli3 could ordinarily perform a role inside the repression of medulloblastoma formation. PE 24. NOVEL CELL LINES FROM PEDIATRIC BRAIN TUMORS Display MULTI DRUG RESISTANCE Jingying Xu, Ignacio Gonzalez Gomez, Elizabeth Y. Melendez, Rex A. Moats, Anat Erdreich Epstein, and C. Patrick Reynolds, Developmental Therapeutics System, USC UCLA Institute for Pediatric Clinical Investigation, Division of Hematology Oncology, Childrens Hospital Los Angeles and Departments of Pediatrics and Pathology, Keck College of Medication, University of Southern California, Los Angeles, CA, USA The paucity of cell culture versions for childhood brain tumors has prompted us to establish this kind of cell lines for use in biological and preclini cal developmental therapeutic scientific studies.
Brain tumors from pediatric sufferers were obtained at surgery or publish mortem and minced and cultured selleck in lscoves Dulbeccos Modified Eagles Medium. Here we describe three new cell lines, CHLA 200, CHLA 259 and CHLA 266. All 3 cell lines had distinctive DNA fingerprints according to the ABI Profiler. All cell lines have been adherent on tissue culture plastic but differed in morphology. The doubling times had been 76 hours for CHLA 259, 45 hours for CHLA 266, and fifty five hrs for CHLA 200 com pared with 29 hours for your commonly utilised medulloblastoma cell line DAOY. In contrast to fibroblasts, these four cell lines expressed telomerase reverse transcriptase and telomerase RNA template.
CHLA 266, CHLA 259, and CHLA 200 strongly expressed the early neuronal marker PGP9. five but did not express tyrosine hydroxylase. DAOY, CHLA 259, and CHLA 266 expressed MYCN mRNA, whereas CHLA 266 and CHLA 200 expressed cmyc. The cell PD318088 lines were injected into the brains of nude mice, but only DAOY and CHLA 266 have confirmed tumorigenic to date. All 3
s showed striking multidrug resistance when in contrast with DAOY cells utilizing a fluorescein diacetate and digital image microscopy cytotoxicity assay. The table displays concentrations of medicines which have been lethal for 90% of cells. CHLA 271 and CHLA 280 are new cell lines. These new brain tumor cell lines will offer helpful models for preclinical testing of novel drugs for doable exercise towards multidrug resistant pediatric brain tumors. This research was supported by the Devin Hock Memorial Fund from the Michael Hoefflin Basis and the Pediatric Brain Tumor Foundation. PE 25. LINEAGE Restricted PROGENITORS CAN SERVE AS CELLS OF ORIGIN FOR MEDULLOBLASTOMA Zeng Jie Yang,1 Tracy Ann Read,1 Tammy Ellis,two Robert Machold,three Gord Fishell,3 David H.