Ased at different speeds may need during the 4 h treatment. In the case of DOX uptake in P388-sensitive cells with drugs or drug-free micelle treated, there is no difference in the absorption of DOX, but in the resistant Integrase cells P388/ADR, micellar DOX treatment of DOX increased Hte uptake compared to free DOX treatment after 1 h of treatment. When the cells were treated with or without PazPC IDA / IDA micelles, the micellar formulation induced h Here uptake of IDA in both sensitive and resistant cells as free IDA treated. However, in received Nglichen cells, the p-values of less than 0.05 for all time points after 2 h of treatment, w While in resistant cells P388/ADR, the values of p less than 0.01 for all time points after 0 , 5 h of treatment. To determine if an improved cellular Re entered admission easy Born from the effects of the lipid, the leukemia Mie cells were treated with drug-free micelles and Virgin PazPC by simply mixing or sequentially. When both cell lines were initially First with empty micelles incubated for 2 h, then washed and with drug free for a further 2 h, the absorption of DOX or IDA has not increased by Hte relative to the free drug. In addition simple mixing of empty micelles with free drug for 2 h only slightly increased Hten drug uptake in sensitive and resistant cell lines. In summary, the induced lligen micellar drug formulations more drug absorption cell without drugs or a simple mixture of medication and empty micelles in leuk Mixing cells of both resistant and anf. In vitro cytotoxicity t of free DOX, IDA, PazPC / DOX, PazPC / IDA micelles and micelles PazPC virgins were carried out in P388 sensitive and resistant cells P388/ADR.
IC50 values are listed in Table 3, and the Lebensf Ability of the cells of both Leuk shown Preconcentrated, purified in. 6th Micelles PazPC / DOX and PazPC / h significantly IDA Here Cytotoxicity t in both cell types compared to free drug. In the case of the resistant cell P388/ADR, the IC50 values of PazPC / DOX micelles 14 times less than that of free DOX and PazPC / IDA micelles were eight times lower than free IDA. However, there were eight times lower IC 50 values for the micelles and DOX 5 times lower on the for the micelles of IDA in cells sensitive P388. Therefore, the cell is more resistant to drugs P388/ADR susceptive PazPC micellar sensitive than their counterparts. More importantly, the drug was micellar PazPC cytotoxicityof in resistant cells comparable to the cytotoxicity t of free drug in sensitive cells, which then causes no IC50 values comparable. Table 3 also shows that the micelles empty PazPC very high IC50 values in the P388 and resistant cells were P388/ADR, and showed no cytotoxicity t on the cell type or the Equivalents of concentration of the drug-loaded micelles PazPC. 4th Discussion concerning the value of the CMC Gt approximately 18.7 PazPC 23.1 million, slightly above what the CMC lipids in the current system of drug delivery micelles, such as PEG 2000 DSPE is used. Interactions involved, hydrophobic and Candesartan electrostatic in the interaction between DOX and PazPC. In the formation of the complex ion pair, the anionic charge of the carboxyl group of the sn 2 of each Of azelaoyl PazPC not neutralized by the charge of the drug. Therefore, the hydrophobic interaction between the aromatic rings of the drug and a sn cha Not intend to force the Sn 2 Cha Do short back and interact spirit.