Mice inoculated with untreated LM8 cells were termed the control group http://www.selleckchem.com/products/Perifosine.html and those inoculated with genistein treated LM8 cells were termed the genistein group. In the control group, all mice exhibited large tumors measuring 1. 6 3. 0 cm at the inoculation site. The engraftment rate of tumor cells, which was calculated by dividing the number of tumor bearing mice by the total number of mice, was 100%. In the genistein group, one mouse did not exhibit tumors at the inoculation site and the remaining seven mice exhibited smaller tumors measuring 0. 6 1. 6 cm compared with the control group. The engraftment rate of tumor cells was 87. 5%. The tumor weight was 3. 85 0. 91 g in the control group and 0. 89 0. 16 g in the genistein group, indicating that genistein treated LM8 cells grew at lower growth rate compared with un treated LM8 cells.

The body weight was 19. 5 1. 0 g in the control group, and 24. 0 0. 7 g in the genistein group. The body weight correlated negatively with the tumor weight. Thus, the body weight decreased with the growth of the primary tumor. To examine the presence of metastatic tumors in nude mice, the sections of formalin fixed, paraffin embedded lungs and livers were stained with H E and observed microscopically under low magnification. In the control group, multiple metastatic tumors were found in the lung and liver and the meta static incidence was 100% in the lung and 87. 5% in the liver. In the genistein group, one exhibited the presence of the metastatic tumor in the liver, but not the lung.

The remaining six mice did not exhibit the presence of any metastatic tumors in the lung or liver, and this group was termed the genistein/metastasis subgroup. The meta static incidence in the genistein group was 0% in the lung and 14. 3% in the liver. In another series of experiments, untreated and genistein treated LM8 cells were subcutaneously inocu lated into the backs of C3H mice. In the control group, all mice exhibited large tumors measuring 0. 7 1. 7 cm at the inoculation site. The en graftment rate of tumor cells was 100%. The tumor weight of this group was 1. 17 0. 20 g. Multiple metastatic nodules were macroscopically identified at the surface of the lung and liver, and the metastatic incidence was 100% in the lung and 57. 1% in the liver. In the genistein group, no mice exhibited any tumors at the inoculation site and developed metastatic nodules at the surface of the lung and liver.

Both the engraftment rate of tumor cells and metastatic incidence were 0%. Expression of B catenin within the primary and metastatic tumors in nude mice The expression of B catenin within the primary tumors was immunohistochemically examined. Positive B catenin immunostaining was predominantly observed in the cytoplasm of tumor Entinostat cells.