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“Nicotinamide (NAM), which is one of the two principal forms, together with nicotinic acid, of vitamin B3, is both a food nutrient and a drug. Controlled NAM release systems are useful to extend the duration of the drug’s pharmacological activity and to minimize administration frequency. In
this paper, molecularly imprinted polymers (MIPs) have been used as unconventional synthetic polymeric carriers, to prepare drug delivery systems for sustained release of NAM molecules. In the present study, various MIPs micro-spheres have been synthesized by using methacrylic acid as a functional monomer and ethylene glycol dimethacrylate (EGDMA) as a cross-linker. Different stoichiometric ratios of the reagents have been used, in order to evaluate their influence on NAM recognition and release properties. Non-imprinted systems have been also been prepared as controls. MIPs binding capacity has been evaluated; https://www.selleckchem.com/products/AZD6244.html NAM loading and in vitro release studies, in buffer solution (pH 7.2), that mimics blood plasma conditions, have been performed. Polymer P4 has given the best results since it enables it to
rebind selectively and to prolong the release of NAM with higher performance than the non-imprinted one.</.”
“BACKGROUND Red light is part of the visible light spectrum. The effects of light-emitting diode (LED)generated red light on human skin are not well-characterized.
OBJECTIVE To study the effect of red LED-generated low-level light therapy Buparlisib supplier (LLLT) on fibroblast proliferation and viability in vitro.
METHODS AND MATERIALS Irradiation of normal human skin fibroblasts using red LED panels was performed in vitro, and modulation of proliferation and viability was quantified using trypan blue dye exclusion assay.
RESULTS Statistically significant decreases in cell proliferation were noted at the following fluences (time): 160 J/cm(2) (30 minutes, 34 seconds), 320 J/cm(2) (61 minutes, 07 seconds) and 640 J/cm(2) (122 minutes, 14 seconds) (Figure 1).
ML323 cell line Irradiation at the 160- (98.5 +/- 1.2%) and 320-J/cm(2) (98.0 +/- 3.1%) doses did not significantly alter viability.
CONCLUSION At certain fluences, red LLLT can effectively inhibit fibroblast proliferation in vitro without altering viability and holds promise for the treatment of scars and other proliferative skin diseases.”
“Objectives: To assess the effect of a training program for smoking cessation combined with chart stickers on resident’s (physicians-in-training) practice of counselling smoking patients.
Setting: A single centre prospective observational study at the Basel University Hospital Medical Outpatient Department.
Methods: 456 consecutive outpatients were contacted by phone within 24 hours of their initial consultation.