Only diamond nanoparticles, multi-wall nanotubes and fullerenes showed statistically significant results. Nanoparticles showing anti-angiogenic effects also changed the morphology of CAM by decreasing its thickness. Diamond nanoparticles and fullerene changed the expression level of KDR, but not #learn more randurls[1|1|,|CHEM1|]# FGFR, thereby affecting the angiogenic potential of CAM. Multi-wall nanotubes and especially diamond nanoparticle can be considered potential inhibitors of blood vessel growth in anti-angiogenic

tumour therapy. Acknowledgements This work was supported by the following grants: NCN 2011/03/N/NZ9/04290 and NCN NN311540840. The report is a part of the doctoral thesis of Mateusz Wierzbicki. References 1. Adams RH, Alitalo K: Molecular regulation of angiogenesis and lymphangiogenesis. Nat Rev Mol Cell Biol 2007, 8:464–478.CrossRef 2. Kurz H, Burri PH, Djonov VG: Angiogenesis and vascular remodeling by intussusception: from form to function. News Physiol Sci 2003, 18:65–70. 3. Ferrara N, Gerber HP, LeCouter J: The biology of VEGF and its receptors. Nat Med 2003, 9:669–676.CrossRef

4. Shibuya M: Differential roles of vascular endothelial growth factor receptor-1 and receptor-2 in angiogenesis. J Biochem Mol Biol 2006, 39:469–478.CrossRef 5. Cross Tanespimycin mw M, Claesson-Welsh L: FGF and VEGF function in angiogenesis: signalling pathways, biological responses and therapeutic inhibition. Trends Pharmacol Sci 2001, why 22:201–207.CrossRef 6. Jain RK, Duda DG, Clark JW, Loeffler JS: Lessons from phase III clinical trials on anti-VEGF therapy for cancer. Nat Clin Pract Oncol 2006, 3:24–40.CrossRef 7. Carmeliet

P, Jain RK: Molecular mechanism and clinical applications of angiogenesis. Nature 2011, 473:298–307.CrossRef 8. Sayes CM, Fortner JD, Guo W, Lyon D, Boyd AM, Ausman KD, Tao YJ, Sitharaman B, Wilson LJ, Hughes JB, West JL, Colvin VL: The differential cytotoxicity of water-soluble fullerenes. Nano Lett 2004, 4:1881–1887.CrossRef 9. Dumortier H, Lacotte S, Pastorin G, Marega R, Wu W, Bonifazi D, Briand JP, Prato M, Muller S, Bianco A: Functionalized carbon nanotubes are non-cytotoxic and preserve the functionality of primary immune cells. Nano Lett 2006, 6:1522–1528.CrossRef 10. Schrand AM, Dai L, Schlager JJ, Hussain SM, Osawa E: Differential biocompatibility of carbon nanotubes and nanodiamonds. Diam Relat Mater 2007, 16:2118–2123.CrossRef 11. Liu KK, Cheng CL, Chang CC, Chao JI: Biocompatible and detectable carboxylated nanodiamond on human cell. Nanotechnology 2007, 18:325102.CrossRef 12. Grodzik M, Sawosz E, Wierzbicki M, Orlowski P, Hotowy A, Niemiec T, Szmidt M, Mitura K, Chwalibog A: Nanoparticles of carbon allotropes inhibit glioblastoma multiforme angiogenesis in ovo. Int J Nanomedicine 2011, 6:3041–3048. 13.