of theActivated neutrophils. R The depolarization of the membrane effective release of Ca 2 through the plasma membrane, and Ca 2 facilitated by the action of membrane depolarization endomembrane ATPases of NADPH oxidase, which limit the influx of Ca 2 +. NADPH oxidase is membrane associated, electron transport compound, wherein the system of phagocytes superoxide generating PD-183805 dependent-Dependent activation of Ca 2 erf Leads w During the exposure of cells to chemotactic cytokines and opsonized antigens. The dramatic decrease in the membrane potential that accompanies the activation of NADPH oxidase and co F falls With the release of intracellular Ca2 neutrophil Ren Save cation efflux and activation of the superoxide production was attributable Oxidaseaktivit t generators and the action of a conductance fast inflow with H Men from H.
This type of membrane depolarization activates pl occurred Tzlich has been shown to limit the influx of Ca 2 +. When cells are depolarized, the driving force for the entry of Ca 2 will be removed, because the electrical component GDC-0879 of the electrochemical gradient is substantially reduced to the entry of Ca 2 F Promotion. Repolarization, and the driven membrane m Possible influx of Ca 2 absorption of Ca 2 fMLP activated neutrophils galvanized in an event Siege, with net contribution is only about 1 min after addition of the chemoattractant and gradually of detectable over a period of 5 min time. This type Ca 2 + influx is characteristic mechanisms operated stores, secondary to the emptying of intracellular fill Ren Ca 2 + stores and the store had.
Interestingly, the evolution in time of the influx of Ca 2 in the repolarization of the membrane, which is superimposed on a mechanical relationship between these two events. It has been suggested that membrane repolarization YEARS in activated eosinophils by NADPH oxidase Ring H channel extrusion, the generators, the results of the repolarization of the cell membrane is taught. Despite the r Crucial to the proton efflux in the regulation of intracellular Ren pH, k can Other mechanisms that contribute to membrane repolarization in activated neutrophils. It is important that the mechanism of charge compensation in dependence Dependence and vary the nature of the signal transduction pathways.
Membrane repolarization of neutrophils with the regulation of the activity of t chemoattractantactivated Generator operating parameters of the plasma membrane Na 2 Ca exchanger in the opposite direction are assigned. Treatment with activated neutrophils KB R7943, a selective inhibitor of the Na Ca W Exchanger, which inhibits reverse mode 2, clearly the rate and extent the recovery of the membrane potential in the direction will rest tte levels. Important is that the inhibition of membrane repolarization of KB R7943 with a significant deviation attenuator The shop Ca2 operated by these cells, indicating that these processes are linked together. R The repolarization of the membrane in the regulation of the rate of the calcium influx is also supported by the observation that The addition of the NADPH oxidase inhibitor, diphenyleneiodonium chloride neutrophils after 1 min FMLP potentiated the rate and extent repolarization, increased at a corresponding rate ht storeoperated