ADHGEPIGR, Including mass spectrometric identification of the peptide of the Lich site supply Hsp90 H442: LGIHEDSTNR, mass spectrometry Identifying a peptide that the place of the Hsp90 H458 TSQSGDEMTSLSEYVSR supply, the mass of the identification PHA-680632 peptide with Hsp90 spectral place adduction H625: KHLEINPDHPIVETLR, the mass of the identification peptide with Hsp90 spectral place adduction H632: Including HLEINPDH PIVETLR, mass spectral identification of the peptide Lich providing siteHsp90RH450 : LGIHEDSQNR, identification of mass spectra of the peptide location includinmg supply Hsp90R H490: HIYYITGETK.  Small regulatory RNAs such as small interfering RNAs and micro RNAs regulate gene expression, especially post-transcriptional level in eukaryotes. Argonauts, small RNA, mRNA and miRNA target has been shown to be in cytoplasmic processing K Localized body.
This structure is confinement in cytoplasmic different mechanisms Lich mRNA degradation are involved, hangs Entsch MAY BE lacing poly AU-rich SGX-523 element mediated mRNA destabilization, and nonsense-mediated mRNA degradation. Argonauts metazoans interact GW182 family of proteins, the first as a marker K Body identified P. Depletion of GW182 family creates visible PK Body, which also leads to a loss of miRNA-mediated repression in reporter constructs, as the Argonauts, the interaction with GW182 for mediating the suppression of the miRNA genes ben CONFIRMS is. Numerous studies have shown that the function of the proteins GW182 Can Be uncoupled in translational repression of the Argonauts, suggesting that miRNA responsible for recruiting Argonauts GW182 proteins Targeted mRNA.
Other proteins, such Mov10 Rck/p54 and the interaction with the Argonauts and play an r Essential role in the suppression of the miRNA genes mediating with the Argonauts in organs P. collocation Rck/p54 st impoverishment Rt and the formation of PK Rpern visible. The presence of microscopic PK Rpern seems not a prerequisite for the thwart of miRNA genes mediation. For example, depletion LSM1, a component of PK rpern St Ugerzellen rt the formation of P bodies in S, But not significantly influenced t-mediated repression of miRNA genes. It was also proposed that the P body formation is a consequence of RNA silencing. Additionally Tzlich recent studies have shown that GW182 function of its location may be uncoupled within the organs P. ben Interestingly CONFIRMS the P body formation, the presence of small regulatory RNAs.
Drosha depletion leads to loss of K Rpers visible P can be reconstituted by the introduction of synthetic siRNA. Introduction of functional siRNA in S ugerzellen That induce up regulation of Ago2 and GW182 K Body P and produce more microscopically. Biochemical purification revealed that Argonaute proteins h Frequently with heat shock proteins copurify. It has been found that heat-shock protein 90 binds to the N-terminus of S Uger Ago2. Overexpressed and proposed is required to interact with Dicer Ago2 stabilize However, very recently, a study has been suggested that HSP90 function by function and RISC showed genetic studies in Arabidopsis that the known binding partners of the HSP90, cyclophilin 40, for the activity of T Required miRNAs required. HSP90 is part of a multi-protein complex Abh-dependent Chaperonaktivit t of ATP.