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immunohistochemical studies have shown that Pick bodies are immunoreactive for synaptic proteins.[29] These findings suggest that the proteins synthesized in neuronal perikarya might be entrapped within the filamentous structure of Pick bodies. However, in the present study Pick bodies present inside and outside the dentate gyrus were intensely immunolabeled with anti-FIG4. Moreover, co-localization of FIG4 and phosphorylated tau was seen in the neuropil, which corresponds to small Pick bodies in the neurites.[27, 28] It seems likely that incorporation of FIG4 into Pick bodies is a pathological event, and does not simply reflect entrapment of the protein. Lewy bodies consist of a dense core and a peripheral halo, which correspond

ultrastructurally to zones of densely PLX-4720 chemical structure compacted circular profiles and zones of filaments, respectively.[30] It is well known that the constituent filaments of Lewy bodies are composed of α-synuclein. However, little is known about the components of the central core of Lewy bodies. In the present study, the cores of brainstem-type and cortical Lewy bodies were immunolabeled intensely by anti-FIG4 antibody, but their peripheral portions were only weakly stained or unstained. This localization implies that FIG4 is involved in formation of the central core of Lewy bodies and that FIG4 may not interact with α-synuclein. In polyglutamine diseases, Lumacaftor order NNIs in DRPLA and SCA3,

but not in HD, SCA1 and SCA2, were immunopositive for FIG4. NNIs in INIBD were also positive for FIG4. In addition to the cytoplasm, FIG4 is reportedly localized in the nuclear pore, being required for efficient export of nuclear signal-containing reporter protein.[31] This interaction is thought to be important for the regulation of gene expression or DNA synthesis.[30] In polyglutamine diseases, NNIs may affect nuclear function and recruitment of other proteins, possibly resulting in loss of the physiological function of recruited proteins, and subsequent neuronal dysfunction.[32] Similar mechanisms may occur in the pathogenesis of INIBD, although the major component of nuclear inclusions in this disease is uncertain. It is possible that FIG4 translocates from the cytoplasm to the Vitamin B12 nucleus in order to protect cells from cytotoxic events. However, it is unclear why only two polyglutamine diseases (DRPLA and SCA3) showed FIG4 immunoreactivity in NNIs. The evidence suggests that the mechanism of inclusion body formation may differ among the various polyglutamine diseases. In the present study, Marinesco bodies were also immunoreactive for FIG4. The frequency of Marinesco bodies is significantly higher in nigral neurons with Lewy bodies than in those without.[33] The melanin content of nigral neurons containing Marinesco bodies is lower than that of nigral neurons lacking Marinesco bodies.