Past reports exposed that chemical ingestion induce suppres sion of sexual habits of male rats and reductions in motility. Antioxidant enzyme perform critical function in oxidative infertil ity. Oxidative anxiety may possibly result in overproduction of oxy gen cost-free radical precursors and or decreased efficiency of the antioxidant technique. CCl4 and oxygen no cost radical generation is associated with impaired glutathione me tabolism, alterations during the antioxidant standing. The outcomes of our present investigation showed that 3 ml kg CCl4 administration in rats triggered substantial reduction inside the action of antioxidant enzymes, GSH and improved TBARS. Reduction of antioxidant enzymes ac tivity in testicular tissue are could be due to accumula tion of free radicals results in enhanced lipid peroxidation or inactivation in the antioxidative enzymes.
Gluta thione contents perform key position in sustaining antioxidant a cool way to improve standing. Decrease in GSH action through CCl4 toxicity could possibly be as a result of decreased availability of GSH resulted throughout the enhanced lipid peroxidation. Im provement of testicular GSH levels in rats treated with Launaea procumbens extracts in comparison to CCl4 ad ministration further demonstrated the antioxidative ef fect from the plant. Many solutions of Launaea procumbens extracts also enhanced the ranges of antioxi dant enzymes in CCl4 administered rats are due the presence of phenolic and polyphenolic constituents which may have unique functional properties like scavenging of active oxygen species, inhibition of the generation of cost-free radicals and chain breaking activity.
Related observations more info here had been also reported with black tea extract around the level of TBARS in rats right after CCl4 expos ure. Jia et al, investigated that oxidative damage can occur in DNA in the course of the peroxidative breakdown of membrane polyunsaturated fatty acids. DNA harm impacts homeostasis of numerous cells leading to induced signal transductions connected with apoptosis and cell proliferation. Administration of Launaea procum bens extracts to CCl4 intoxicated rats protected and markedly decreased the percentage of fragmented DNA that was also exposed in DNA ladder assay. It could con tribute its protective results by erasing the damaging ac tion of CCl4 at DNA level. The protective probable might both involve antioxidant, signal transduction, gene ex pression, and effective involvement inside the metabolic pathways.
Histopathalogical research revealed that CCl4 treatment showed marked degeneration and alterations of germ cells, nonetheless therapy of Launaea procumbens showed obvious improvement in histopathalogical modifications induced by CCl4 in testis sections. The histo logical alterations in testes of rats administered CCl4 are in agreement with Khan and Ahmed who studied the result of Digera muricata towards CCl4 induced tox icity over the rats testes.