PubMedCrossRef 43. Bowen WH, Schilling K, Giertsen E, Pearson S, Lee SF, Bleiweis A, et al.: Role of a cell surface-associated protein in adherence and dental caries. Infect Immun 1991, 59:4606–4609.PubMed 44. Takao A, Nagamune H, Maeda N: Sialidase of Streptococcus intermedius : a putative virulence factor modifying

sugar chains. Microbiol Immunol 2010, 54:584–595.PubMed 45. McEllistrem MC, Ransford JC, Khan SA: Characterisation of in vitro biofilm-associated pneumococcal phase variants of a clinically-relevant serotype 3 clone. J Clin Microbiol 2007, 45:97–101.PubMedCrossRef 46. Branda SS, Vik S, Friedman L, Kolter R: Biofilms: the matrix revised. Trends Microbiol 2005, 13:20–26.PubMedCrossRef mTOR inhibitor 47. Pearce BJ, Iannelli F, Pozzi G: Construction of new unencapsulated (rough) strains of Streptococcus pneumoniae . Res Microbiol 2002, 153:243–247.PubMedCrossRef selleck inhibitor 48. Iannelli F, Pozzi G: Method for introducing specific and unmarked mutations into the chromosome of Streptococcus pneumoniae . Mol Biotechnol 2004, 26:81–86.PubMedCrossRef 49. Throup JP, Koretke KK, Bryant AP, Ingraham

KA, Chalker AF, Ge Y, et al.: A genomic analysis of two-component signal transduction in Streptococcus pneumoniae . Mol Microbiol 2000, 35:566–576.PubMedCrossRef 50. Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001, 25:402–408.PubMedCrossRef 51. Schmittgen TD, Livak KJ: Analyzing real-time

PCR data by the comparative C(T) method. Nat Protoc 2008, 3:1101–1108.PubMedCrossRef 52. Tettelin H, Nelson KE, Paulsen IT, Eisen JA, Read TD, Peterson S, et al.: Complete genome sequence of a virulent isolate of Streptococcus pneumoniae . Science 2001, 293:498–506.PubMedCrossRef 53. Iannelli F, Chiavolini D, Ricci S, Oggioni MR, Pozzi G: Pneumococcal surface protein C (PspC) contributes to sepsis caused by Streptococcus pneumoniae . Infect Immun 2004, 72:3077–3080.PubMedCrossRef 54. Iannelli F, Pearce BJ, Pozzi G: The type 2 capsule locus of Streptococcus pneumoniae . J Bacteriol 1999, 81:2652–2654. 55. Oggioni MR, Memmi G, Maggi T, Chiavolini D, Iannelli F, Pozzi G: Pneumococcal zinc metalloproteinase ZmpC cleaves human matrix metalloproteinase Cytidine deaminase 9 and is a virulence factor in experimental pneumonia. Mol Microbiol 2003, 49:795–805.PubMedCrossRef 56. Romao S, Memmi G, Oggioni MR, Trombe MC: LuxS impacts on CUDC-907 datasheet lytA-dependent autolysis and on competence in Streptococcus pneumoniae . Microbiology 2006, 152:333–341.PubMedCrossRef Authors’ contributions CT preformed experiments of microtiter biofilm model 1. LG set up microtiter biofilm model 2. DML performer the experiments of microtiter biofilm model 2. PJ performer experiments on continuous culture biofilm. CCK performer experiments on continuous culture biofilm. PE supervised the continuous culture biofilm and particpated in writing of the manuscript. FI supervised and performer construction of mutant.