We therefore analyzed the effect of overepressing PreA in a ΔpreA strain carrying preA driven by a pBAD arabinose-inducible promoter grown in buffered LB. In addition, past experiments had implied that PreB may be acting as a protein phosphatase

when bacteria are grown in LB [3]. If this is the case, some of the regulatory effects attributed to preA may have been dampened in the previous experimental design. We therefore proceeded to also analyze the cDNA from a preAB double mutant expressing pBAD-preA and a preAB strain carrying the vector control. All of the data from both experiments is included in Additional file 1, {Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|buy Anti-infection Compound Library|Anti-infection Compound Library ic50|Anti-infection Compound Library price|Anti-infection Compound Library cost|Anti-infection Compound Library solubility dmso|Anti-infection Compound Library purchase|Anti-infection Compound Library manufacturer|Anti-infection Compound Library research buy|Anti-infection Compound Library order|Anti-infection Compound Library mouse|Anti-infection Compound Library chemical structure|Anti-infection Compound Library mw|Anti-infection Compound Library molecular weight|Anti-infection Compound Library datasheet|Anti-infection Compound Library supplier|Anti-infection Compound Library in vitro|Anti-infection Compound Library cell line|Anti-infection Compound Library concentration|Anti-infection Compound Library nmr|Anti-infection Compound Library in vivo|Anti-infection Compound Library clinical trial|Anti-infection Compound Library cell assay|Anti-infection Compound Library screening|Anti-infection Compound Library high throughput|buy Antiinfection Compound Library|Antiinfection Compound Library ic50|Antiinfection Compound Library price|Antiinfection Compound Library cost|Antiinfection Compound Library solubility dmso|Antiinfection Compound Library purchase|Antiinfection Compound Library manufacturer|Antiinfection Compound Library research buy|Antiinfection Compound Library order|Antiinfection Compound Library chemical structure|Antiinfection Compound Library datasheet|Antiinfection Compound Library supplier|Antiinfection Compound Library in vitro|Antiinfection Compound Library cell line|Antiinfection Compound Library concentration|Antiinfection Compound Library clinical trial|Antiinfection Compound Library cell assay|Antiinfection Compound Library screening|Antiinfection Compound Library high throughput|Anti-infection Compound high throughput screening| but a focused list of key candidate regulated genes is shown in Table 2. Table 2 Microarray and real time PCR analysis showing a limited list of genesa predicted to be PreAB activated ORF Gene Function Microarray Ab Md (fold change) Microarray Bc M (fold change) qRT-PCRe STM3707 yibD putative glycosyltransferase 0.8 (1.7) 6.1 (68.6) NP f STM3176 ygiW Membrane protein (DUF388; exporter?) 4.5 (22.6) 5.2 Selleckchem NVP-BSK805 (36.8) 355 STM1253   Cytochrome b561 (Ni2+ dependent) 2.9 (7.5) 4.9 (29.9) 372 STM1595 srfC ssrAB activated gene; predicted coiled-coil structure 4.3 (19.7) 4.7 (26.0) 1.2 STM3175   putative bacterial regulatory helix-turn-helix proteins,

AraC family 3.6 (12.1) 4.4 (21.1) 605.3 STM1685 ycjX putative ATPase 2.3 (4.9) 3.8 (13.9) 37.7 STM1252   putative cytoplasmic protein 1.5 (2.8) 2.8 (7.0) 8.6 STM3179 mdaB NADPH specific quinone oxidoreductase (drug modulator) 1.0 (2.0) 2.8 (7.0) 32.5 STM1684 ycjF putative inner membrane

protein 1.1 (2.1) 2.6 (6.1) 61.2 STM4291 pmrB sensory kinase in buy FG-4592 two-component regulatory system with PmrA ND g 2.1 (4.3) NP STM2080 udg UDP-glucose/GDP-mannose dehydrogenase ND 1.8 (3.5) 23.2 STM4292 pmrA response regulator in two-component regulatory system with PmrB ND 1.7 (3.2) NP STM4118 yijP (cptA) putative integral membrane protein ND 1.5 (2.8) 32.8 STM0628 pagP PhoP-activated gene, palmitoyl transferase ND 1.1 (2.1) NP STM2238   putative phage protein 0.9 (1.9) 1.0 check details (2.0) NP a This list includes only those genes that were upregulated in both the preA and preAB mutant strains overexpressing preA, those confirmed by real-time PCR, genes previously shown to be preA-regulated (yibD, pmrAB) or those known to belong to the PhoPQ or PmrAB regulons b ΔpreA/pBAD18-preA vs. ΔpreA/pBAD18 c ΔpreAB/pBAD18-preA vs. ΔpreAB/pBAD18 d M = Log2(expression plasmid/vector control) e real time PCR (qRT-PCR) performed with cDNA derived from the strains used in Microarray B f NP = not performed g ND = not detected Many of the genes upregulated in the ΔpreA strain overexpressing preA (Table 2, column 1) were reconfirmed in experiments with the preAB mutant strain overexpressing preA (Table 2, column 2), but with increased fold activation.