Multivariate analysis statistically separated sample grids from infested GSK2245840 mw and uninfested areas. Following treatment, no P. megacephala individuals were detected for 2 years and it was therefore declared eradicated. Ecological recovery post treatment was also clear. Twenty-one months post-treatment, native ant abundance and species richness within the treated (infested) area were always almost always significantly greater than in the pre-treatment

sample, corresponding with no change in the control area (uninfested area). Total species richness from plots in the treated area was identical to that from plots in the control area. Multivariate analysis showed no statistical separation of the treated or control plots. Species richness within lure plots displayed no trend within the treated area relative to the treatment boundary or locations away from the treated area. This project demonstrates the feasibility GSI-IX of eradicating this ant, and that ecological systems are capable of recovering following removal of an exotic invader.”
“The common killifish or mummichog (Fundulus heteroclitus) is an estuarine teleost increasingly used in comparative physiology, toxicology and embryology. Their ability to withstand extreme environmental conditions and ease

of maintenance has made them popular aquatic research organisms. Scientific advances with most popular model organisms have been assisted with the availability of continuous cell lines; however, cell lines from F. heteroclitus appear to be unavailable. The development of a killifish cell line, KFE-5, derived from the mid trunk region of a late stage embryo is described here. KFE-5 grows well in GM6001 Leibovitz’s L-15 media with 10% fetal bovine serum (FBS). This cell line has been passaged over 60 times in a span of three years, and cells at various passages have been successfully cryopreserved and thawed. The cells are mostly fibroblastic but contain myogenic cells that differentiate into mono-, bi- and multi-nucleated

striated myocytes. Immunofluorescence detection of muscle specific antigens such as alpha-actinin, desmin, and myosin confirms KFE-5 as a myogenic cell line. KFE-5 has a temperature preference for 26-28 degrees C and has been shown to withstand temperatures up to 37 degrees C. The cell line responds to chemical signals including growth factors, hormones and extracellular matrix components. KFE-5 could thus be useful not only for mummichog’s thermobiology but also for studies in fish muscle physiology and development. (C) 2014 Elsevier Inc. All rights reserved.”
“Objective: Hepatocellular carcinoma (HCC) staging systems were developed using data predominantly from patients who had hepatitis and cirrhosis.