Upon her return to Oxford from her USA sabbatical she also studied the patterns of RNA metabolism in the different functional states of bone cells. These investigations of the dynamics of cell differentiation and the hormonal effects on bone were investigated in great detail, by the tedious method of cell and autoradiographic grain Selumetinib cost counting, in ground-breaking publications. In particular the effects of parathyroid extract were shown in vivo on both mature osteoblasts and osteoclasts and also on osteogenic and osteoclastic bone progenitor

cellular activity. An important observation was that the there were different and rapid effects of the hormone on uptake of RNA precursors in the osteoblasts and osteoclasts. In Linsitinib cell line addition to her studies with radioactively labelled amino acids, labelled glucosamine as a precursor of glycoproteins was used to show the autoradiographic distribution with time of labelled material found associated with osteoclasts and the resorbing bone surfaces. The osteoclast was suggested to synthesise and subsequently to extrude such labelled material on the resorbing bone surface to aid resorption. This uptake was enhanced by parathyroid hormone. Another important outcome from her work was the observation that the high

labelling by thymidine of the ‘preosteoblastic layer’ on the periosteal surfaces of the young rabbits studied, most probably was in a layer immediately adjacent to the local stem cells of this tissue from which other cells are derived. In addition the cell kinetics of the fibroblast–pre-osteoblast–osteoblast–osteocyte system was investigated and this generated many important conclusions concerning cell transitions through these

compartments and functional matrix synthesis. At that time, however, in the early 1960s, the osteogenic and osteoclastic cell lineages were considered to be different functional states of the same cell rather than having distinctly separate origins postnatally, as was proven later. Following Dame Janet’s retirement Maureen, now a permanent member of the MRC External Scientific Staff, succeeded her as head of the newly-named MRC Bone Research Laboratory firstly at the Churchill Hospital Oxford, and subsequently, DAPT solubility dmso in 1974, at the Nuffield Orthopaedic Centre. At this point in her career her focus on osteoblastic cell differentiation became paramount. With remarkable insight, she recognised that the progenitor cells of musculoskeletal stromal tissues would be central to future investigations of bone diseases and their treatments and to normal musculoskeletal physiology. In an editorial in Calcified Tissue Research in 1978, Maureen drew attention to the importance of the two separate cell systems present in bone marrow, the stromal and haemopoietic systems.